Mouse KC ELISA

$398.00
ELM-KC-1

RayBio® Mouse KC (CXCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 150 pg/ml
Recommended Dilution (serum & plasma)
3 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
Growth-regulated alpha protein (C-X-C motif chemokine 1) (Platelet-derived growth factor-inducible protein KC) (Secretory protein N51)
Accession Number
Gene Names
Cxcl1, Gro, Gro1, Mgsa, Scyb1
Gene ID
14825

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CXCL1

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Kumar M., Sahu S., Kumar R., et al. MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-κ Pathway. Cell Host Microbe. 2015 Mar 11;17(3):345-56. doi: 10.1016/j.chom.2015.01.007.
    Species: Mouse
    Sample Type: Conditioned Media

  2. Chan K., Pillon N., Sivaloganathan D., et al. Palmitoleate Reverses High Fat-induced Proinflammatory Macrophage Polarization via AMP-activated Protein Kinase (AMPK). J Biol Chem. 2015 Jul 3;290(27):16979-88. doi: 10.1074/jbc.M115.646992.
    Species: Mouse
    Sample Type: Conditioned Media

  3. Evans H., Killoran K., Mitre B., et al. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice. J Immunol. 2015 Oct 1;195(7):2973-84. doi: 10.4049/jimmunol.1500081.
    Species: Mouse
    Sample Type: Conditioned Media

  4. Brown K., et al. Tumor Necrosis Factor induces developmental stage-dependent structural changes in the immature small intestine. Mediators of Inflammation, Received 13 June 2014; Revised 29 July 2014; Accepted 31 July 2014
    Species: Mouse
    Sample Type: Tissue Lysate

  5. Mouzaoui S., et al. Tumor Necrosis Factor-alpha-Induced Colitis Increases NADPH Oxidase 1 Expression, Oxidative Stress, and Neutrophil Recruitment in the Colon: Preventive Effect of Apocynin
    Species: Mouse
    Sample Type: Tissue Lysate

  6. Liu Z, Petersen R, Devireddy L. Impaired neutrophil function in 24p3 null mice contributes to enhanced susceptibility to bacterial infections. J Immunol. 2013 May 1;190(9):4692-4706.
    Species: Mouse
    Sample Type: Peritoneal Fluid

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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