Mouse Leptin ELISA

RayBio® Mouse Leptin ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Old price: $723.60
$578.88  
You save: $144.72 (20%)
ELM-Leptin-2
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Assay Format

Detection Range:
4 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
10 - 40 fold
Sensitivity:
4 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF- 1alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P41160
Gene ID:
  • 16846
Gene Symbols:
  • LEP
  • OB
Protein Name & Synonyms:
Leptin (Obesity factor)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Li E., Shan H., Chen L., et al. OLFR734 Mediates Glucose Metabolism as a Receptor of Asprosin. Cell Metab . 2019 Aug 6;30(2):319-328.e8. doi: 10.1016/j.cmet.2019.05.022. Epub 2019 Jun 20.
    Species: Mouse
    Sample type: Plasma (Asprosin in glucose metabolism mouse model)
  2. Wang X-A, Zhang R, Jiang D, et al. Interferon regulatory factor 9 protects against hepatic insulin resistance and steatosis in male mice. Hepatology. 2013 Aug;58(2):603-616.
    Species: Mouse
    Sample type: Serum
  3. Qian S-W, Tang Y, Li x, et al. BMP4-mediated brown fat-like changes in white adipose tissue alter glucose and energy homeostasis. Proc Natl Acad Sci USA. 2013 Feb 26;110(9):E798-807.
    Species: Mouse
    Sample type: Serum
  4. Martín R, Hernández M, Córdova C, Nieto M. Natural triterpenes modulate immune-inflammatory markers of experimental autoimmune encephalomyelitis: therapeutic implications for multiple sclerosis. British Journal of Pharmacology 2012;166(5):1708-1723. doi:10.1111/j.1476-5381.2012.01869.x.
    Species: Mouse
    Sample type: Serum (EAE)
  5. Martín R, Hernández M, Córdova C, Nieto M. Natural triterpenes modulate immune-inflammatory markers of experimental autoimmune encephalomyelitis: therapeutic implications for multiple sclerosis. British Journal of Pharmacology 2012;166(5):1708-1723. doi:10.1111/j.1476-5381.2012.01869.x.
    Species: Mouse
    Sample type: Tissue Lysate (Spinal Cord Samples, EAE)
  6. Park J., Shim H., Na A., et al. Orexin A regulates plasma insulin and leptin levels in a time-dependent manner following a glucose load in mice. Diabetologia. 2015 Jul;58(7):1542-50. doi: 10.1007/s00125-015-3573-0.
    Species: Mouse
    Sample type: Plasma (Glucose Tolerance testing of high fat diet C57BL/6 mice)
  7. Naville, Danielle, et al. "Low-dose food contaminants trigger sex-specific, hepatic metabolic changes in the progeny of obese mice." The FASEB Journal 27.9 (2013): 3860-3870.
    Species: Mouse
    Sample type: Plasma (obese mice)
  8. Wang, Xin-An, et al. "CARD3 deficiency exacerbates diet-induced obesity, hepatosteatosis, and insulin resistance in male mice." Endocrinology 154.2 (2013): 685-697.
    Species: Mouse
    Sample type: Serum (CARD3-knockout (KO) and wild-type (WT) mice fed a high-fat diet (HFD) to study obesity/insulin resistance)
  9. Garg, S., et al. "Intervention of probiotic L. reuteri fermented milk as an adjuvant to combat protein energy malnourishment induced gut disturbances in albino mice." Journal of Functional Foods 36 (2017): 467-479.
    Species: Mouse
    Sample type: Serum
  10. Wang, Xin-An, et al. "Interferon regulatory factor 7 deficiency prevents diet-induced obesity and insulin resistance." American Journal of Physiology-Endocrinology and Metabolism 305.4 (2013): E485-E495.
    Species: Mouse
    Sample type: Serum (C57BL/6 and IRF7 knockout mice - diet induced insulin resistance obesity model)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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