RayBio® Mouse MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

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Antigen Information

Accession Number:
  • P10148
Gene ID:
  • 20296
Gene Symbols:
  • CCL2
  • JE
  • MCP1
  • SCYA2
Protein Name & Synonyms:
C-C motif chemokine 2 (Monocyte chemoattractant protein 1) (Monocyte chemotactic protein 1) (MCP-1) (Platelet-derived growth factor-inducible protein JE) (Small-inducible cytokine A2)
Target Species:
  • Mouse

Assay Format

Detection Range:
3 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
3 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Shoulders H., Farner K., Singla D. Macrophage depletionbyclondronate attenuates bonemorphogeneticprotein-7 induced M2macrophagedifferentiationand improved systolicbloodvelocityin atherosclerosis. Translational ResearchVolume 203, January 2019, Pages 1-14, in press
    Species: Serum
    Sample type: Serum
  2. Nandi, Ajeya, and Biswadev Bishayi. "A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1."?Immunology letters?190 (2017): 93-107.
    Species: Mouse
    Sample type: Cell lysates
  3. Nandi A., Bishayi B. Murine macrophage response from peritoneal cavity requires signals mediated by chemokine receptor CCR-2 during Staphylococcus aureus infection. Immunol Res. 2016 Feb;64(1):213-32. doi: 10.1007/s12026-015-8739-9.
    Species: Mouse
    Sample type: Conditioned Media
  4. Calvayrac O., Rodriguez-Calvo R., Marti-Pamies I., et al. NOR-1 modulates the inflammatory response of vascular smooth muscle cells by preventing NF?B activation. J Mol Cell Cardiol. 2015 Mar;80:34-44. doi: 10.1016/j.yjmcc.2014.12.015
    Species: Mouse
    Sample type: Serum
  5. Spreadbury I., Ochoa-Cortes F., Ibeakanma C., et al. Concurrent psychological stress and infectious colitis is key to sustaining enhanced peripheral sensory signaling. Neurogastroenterol Motil. 2015 Mar;27(3):347-55. doi: 10.1111/nmo.12497.
    Species: Mouse
    Sample type: Conditioned Media
  6. Vergori L., Lauret E., Gaceb A., et al. PPAR? Regulates Endothelial Progenitor Cell Maturation and Myeloid Lineage Differentiation Through a NADPH Oxidase-Dependent Mechanism in Mice. Stem Cells. 2015 Apr;33(4):1292-303. doi: 10.1002/stem.1924.
    Species: Mouse
    Sample type: Conditioned Media
  7. Singla D., Singla R., Abdelli L., Glass C. Fibroblast growth factor-9 enhances M2 macrophage differentiation and attenuates adverse cardiac remodeling in the infarcted diabetic heart. PLoS One. 2015 Mar 13;10(3):e0120739. doi: 10.1371/journal.pone.0120739
    Species: Mouse
    Sample type: Tissue Lysate
  8. Carceller M., Guillen M., Ferrandiz M., Alcaraz M. Paracrine in vivo inhibitory effects of adipose tissue-derived mesenchymal stromal cells in the early stages of the acute inflammatory response. Cytotherapy. 2015 Sep;17(9):1230-9. doi: 10.1016/j.jcyt.2015.06.001.
    Species: Mouse
    Sample type: Mouse Air Pouch
  9. Chen M., Zhang J., Hu F., et al. Metformin affects the features of a human hepatocellular cell line (HepG2) by regulating macrophage polarization in a co-culture microenviroment. Diabetes Metab Res Rev. 2015 Nov;31(8):781-9. doi: 10.1002/dmrr.2761
    Species: Mouse
    Sample type: Conditioned Media
  10. Bruce A., Ilagan R., Guthrie K., et al. Selected renal cells modulate disease progression in rodent models of chronic kidney disease via NF-?B and TGF-?1 pathways. Regenerative Medicine October 2015 ,Vol. 10, No. 7, Pages 815-839 , DOI 10.2217/rme.15.43 (doi:10.2217/rme.15.43)
    Species: Mouse
    Sample type: Urine

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