Mouse MIP-2 ELISA

RayBio® Mouse MIP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-MIP2-1
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Antigen Information

Accession Number:
  • P10889
Gene ID:
  • 20310
Gene Symbols:
  • CXCL2
  • MIP-2
  • MIP2
  • SCYB2
Protein Name & Synonyms:
C-X-C motif chemokine 2 (Macrophage inflammatory protein 2) (MIP2)
Target Species:
  • Mouse

Assay Format

Detection Range:
10 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
10 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kumar M., Sahu S., Kumar R., et al. MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-kB Pathway. Cell Host Microbe. 2015 Mar 11;17(3):345-56. doi: 10.1016/j.chom.2015.01.007.
    Species: Mouse
    Sample type: Conditioned Media
  2. Evans H., Killoran K., Mitre B., et al. Ten Weeks of Infection with a Tissue-Invasive Helminth Protects against Local Immune Complex-Mediated Inflammation, but Not Cutaneous Type I Hypersensitivity, in Previously Sensitized Mice. J Immunol. 2015 Oct 1;195(7):2973-84. doi: 10.4049/jimmunol.1500081.
    Species: Mouse
    Sample type: Conditioned Media
  3. Shanmugam A., Wang J., Markand S., et al. Sigma receptor 1 activation attenuates release of inflammatory cytokines MIP1?, MIP2, MIP3?, and IL12 (p40/p70) by retinal Müller glial cells. J Neurochem. 2014 Dec 1. doi: 10.1111/jnc.13002
    Species: Mouse
    Sample type: Conditioned Media
  4. Xu X., Shao B., Wang R., Zhou S., Tang Z., Lu W., Xiong S. Role of Interleukin-17 in defense against pseudomonas aeruginosa infection in lungs. Int J Clin Exp Med 2014;7(4):809-816
    Species: Mouse
    Sample type: BAL Fluid
  5. Liu Z, Petersen R, Devireddy L. Impaired neutrophil function in 24p3 null mice contributes to enhanced susceptibility to bacterial infections. J. Immunol. 2013 May 1;190(9):4692-4706.
    Species: Mouse
    Sample type: Peritoneal Fluid
  6. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Tissue Lysate
  7. Gupta, Ravi Kumar, Sanjay Chhibber, and Kusum Harjai. "Quorum sensing signal molecules cause renal tissue inflammation through local cytokine responses in experimental UTI caused by Pseudomonas aeruginosa." Immunobiology 218.2 (2013): 181-185.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Stogsdill, Megan P., et al. "Conditional overexpression of receptors for advanced glycation end-products in the adult murine lung causes airspace enlargement and induces inflammation." American journal of respiratory cell and molecular biology 49.1 (2013): 128-134.
    Species: Mouse
    Sample type: BALF
  9. Ramachandran, Mohanraj, et al. "An infection-enhanced oncolytic adenovirus secreting H. pylori neutrophil-activating protein with therapeutic effects on neuroendocrine tumors." Molecular Therapy 21.11 (2013): 2008-2018.
    Species: Mouse
    Sample type: Plasma
  10. Oh S, Woo J-I, Lim DJ, Moon SK. ERK2-Dependent Activation of c-Jun is Required for Nontypeable H. influenzae-Induced CXCL2 Up-Regulation in Inner Ear Fibrocytes. Journal of Immunology (Baltimore, Md.?: 1950) 2012;188(7):3496-3505. doi:10.4049/jimmunol.1103182.
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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