Mouse MMP-2 ELISA

RayBio® Mouse MMP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,608.00  
ELM-MMP2-5
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Assay Format

Detection Range:
25 pg/ml - 20000 pg/ml
Recommended Dilution (serum & plasma):
100 - 1,000 fold
Sensitivity:
25 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, MMP-3, proMMP-9, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P33434
Gene ID:
  • 17390
Gene Symbols:
  • MMP2
Protein Name & Synonyms:
72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) [Cleaved into: PEX]
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Sun S., Yu H., Yu H., Honglin M., Ni W., Zhang Y., et al. Inhibition of the Activation and Recruitment of Microglia-LikeCells Protects Against Neomycin-Induced Ototoxicity. Mol Neurobiol DOI 10.1007/s12035-014-8712-y [Epub ahead of print]
    Species: Mouse
    Sample type: Tissue Lysate (Mouse Cochlear basilar membranes)
  2. Chen, Wenxing, et al. "Anti-angiogenesis effect of essential oil from Curcuma zedoaria in vitro and in vivo." Journal of ethnopharmacology 133.1 (2011): 220-226.
    Species: Mouse
    Sample type: Serum ( )
  3. Karampoor S, Zahednasab H, Amini R, Esghaei M, Sholeh M, Keyvani H. Maraviroc attenuates the pathogenesis of experimental autoimmune encephalitis. Int Immunopharmacol. 2020 Mar;80:106138. doi: 10.1016/j.intimp.2019.106138. Epub 2020 Jan 30. PMID: 32007705.
    Species: Mouse
    Sample type: Serum
  4. Thiyagarajan D, Fismen S, Seredkina N, et al. Silencing of Renal DNaseI in Murine Lupus Nephritis Imposes Exposure of Large Chromatin Fragments and Activation of Toll Like Receptors and the Clec4e. Means T, ed. PLoS ONE. 2012;7(3):e34080. doi:10.1371/journal.pone.0034080.
    Species: Mouse
    Sample type: Serum (BW, NZBxNZW nephritis Mice)
  5. Xu J., Ehrman B., Graham L., et al. Interleukin-5 is a potential mediator of angiotensin II-induced aneurysm formation in apolipoprotein E knockout mice. J Surg Res. 2012 Nov;178(1):512-8. doi: 10.1016/j.jss.2011.12.016.
    Species: Mouse
    Sample type: Cell Lysate (Mouse aortic smooth muscle cells)
  6. Xu J., Ehrman B., Graham L., et al. Interleukin-5 is a potential mediator of angiotensin II-induced aneurysm formation in apolipoprotein E knockout mice. J Surg Res. 2012 Nov;178(1):512-8. doi: 10.1016/j.jss.2011.12.016.
    Species: Mouse
    Sample type: Tissue Lysate (Mouse aorta samples from ApoE KO mice)
  7. Yang Y., Wang F., Zhou W., et al. TNF-? stimulates MMP-2 and MMP-9 activities in human corneal epithelial cells via the activation of FAK/ERK signaling. Ophthalmic Res. 2012;48(4):165-70. doi: 10.1159/000338819.
    Species: Mouse
    Sample type: Tissue Lysate (Corenal Lysates following TNFa stimulation)
Worked really well with mouse endothelial cells (cell supernatant). Quick and easy to do.

Yale University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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