Mouse OPG ELISA

RayBio® Mouse Osteoprotegerin (TNFRSF11B) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$1,608.00  
ELM-OPG-5
+

Assay Format

Detection Range:
1 pg/ml - 1200 pg/ml
Recommended Dilution (serum & plasma):
20 - 200 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • O08712
Gene ID:
  • 18383
Gene Symbols:
  • OCIF
  • OPG
  • TNFRSF11B
Protein Name & Synonyms:
Tumor necrosis factor receptor superfamily member 11B (Osteoclastogenesis inhibitory factor) (Osteoprotegerin)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Fradet, Anaïs, et al. "Dual function of ERR? in breast cancer and bone metastasis formation: implication of VEGF and osteoprotegerin." Cancer research 71.17 (2011): 5728-5738.
    Species: Mouse
    Sample type: Conditioned Media (BO2-CT(1/2), BO2- ERRa-WT-1, and BO2-FRT-ERRaDAF2)
  2. Chanda D, Isayeva T, Kumar S, Siegal GP, Szafran AA, et al. Systemic Osteoprotegerin Gene Therapy Restores Tumor-induced Bone Loss in a Therapeutic Model of Breast Cancer Bone Metastasis. Mol Therap. 2008;16(5):871-878. doi:10.1038/mt.2008.48
    Species: Mouse
    Sample type: Serum
  3. Carbonneau, C., Despars, G. et al. Heterotopic bone formation derived from multipotent stromal cells is not inhibited in aged mice. Cytotherapy 2014. Epub Ahead of Print. DOI: 10.1016/j.jcyt.2014.03.004
    Species: Mouse
    Sample type: Conditioned Media (Bone marrow-derived MSCs and osteoblast-like bone stromal cells)
  4. Celik M., Gur A., Akdag Z., et al. The effects of long-term exposure to extremely low-frequency magnetic fields on bone formation in ovariectomized rats. Bioelectromagnetics. 2012 Oct;33(7):543-9. doi: 10.1002/bem.21725.
    Species: Mouse
    Sample type: Serum (Low frequency magnetic exposure in bone formation model)
  5. Hartwich JE, Orr WS, Ng CY, et al. Rapamycin increases neuroblastoma xenograft and host stromal derived osteoprotegerin inhibiting osteolytic bone disease in a bone metastasis model. J Pediatr Surg. 2013 Jan;48(1):47-55.
    Species: Mouse
    Sample type: Serum (xenografted human tumors into CB-17 SCID mice)
  6. Hinenoya H, Katsuyama H, Nohno T. Genistein affects osteoblastic MC3T3-E1 cells both through estrogen receptor and BMP-Smad signaling pathways. Kawasaki Med J. 2013;39(1):21-31.
    Species: Mouse
    Sample type: Conditioned Media (MC3T3-E1 cells)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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