Mouse RANTES ELISA

$398.00
ELM-RANTES-1

RayBio® Mouse RANTES (CCL5) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma)
3 - 30 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
C-C motif chemokine 5 (MuRantes) (SIS-delta) (Small-inducible cytokine A5) (T-cell-specific protein RANTES)
Accession Number
Gene Names
Ccl5, Scya5
Gene ID
20304

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CCL5

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Escudero Paula, Martinez de Marañón Aranzazu, Collado Aida, Gonzalez-Navarro Herminia, Hermenegildo Carlos, Peiró Concepción, Piqueras Laura, and Sanz Maria-Jesus. Antioxidants & Redox Signaling. April 10, 2015, 22(11): 901-920. doi:10.1089/ars.2014.5969.
    Species: Mouse
    Sample Type: Conditioned Media

  2. Escudero P., de Maranon M., Collade A., et al. Combined sub-optimal doses of rosuvastatin and bexarotene impair angiotensin II-induced arterial mononuclear cell adhesion through inhibition of Nox5 signaling pathways and increased RXR/PPARα and RXR/PPARγ interactions.. Antioxid Redox Signal. 2015 Apr 10;22(11):901-20. doi: 10.1089/ars.2014.5969.
    Species: Mouse
    Sample Type: Conditioned Media

  3. Bruce A., Ilagan R., Guthrie K., et al. Selected renal cells modulate disease progression in rodent models of chronic kidney disease via NF-κ and TGF-β1 pathways. Regenerative Medicine October 2015 ,Vol. 10, No. 7, Pages 815-839 , DOI 10.2217/rme.15.43 (doi:10.2217/rme.15.43)
    Species: Mouse
    Sample Type: Urine

  4. Koppe U., Hogner K., Doehn JM., et al. Streptococcus pneumoniae stimulates a STING- and IFN regulatory factor 3-dependent type I IFN production in macrophages, which regulates RANTES production in macrophages, cocultured alveolar epithelial cells, and mouse lungs. J Immunol. 2012 Jan 15;188(2):811-7. doi: 10.4049/jimmunol.1004143.
    Species: Mouse
    Sample Type: Conditioned Media

  5. Ko G., Linfer D., Jang H., et al. Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5. Am J Physiol Renal Physiol. 2012 Mar 15;302(6):F762-73. doi: 10.1152/ajprenal.00335.2011.
    Species: Mouse
    Sample Type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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