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Antigen Information
Accession Number:
- P30882
Gene ID:
- 20304
Gene Symbols:
- CCL5
- SCYA5
Protein Name & Synonyms:
C-C motif chemokine 5 (MuRantes) (SIS-delta) (Small-inducible cytokine A5) (T-cell-specific protein RANTES)
Target Species:
- Mouse
Assay Format
Detection Range:
1 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma):
3 - 30 fold
Sensitivity:
1 pg/ml
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Specificity:
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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Escudero Paula, Martinez de Marañón Aranzazu, Collado Aida, Gonzalez-Navarro Herminia, Hermenegildo Carlos, Peiró Concepción, Piqueras Laura, and Sanz Maria-Jesus. Antioxidants & Redox Signaling. April 10, 2015, 22(11): 901-920. doi:10.1089/ars.2014.5969.
Species: Mouse
Sample type: Conditioned Media -
Escudero P., de Maranon M., Collade A., et al. Combined sub-optimal doses of rosuvastatin and bexarotene impair angiotensin II-induced arterial mononuclear cell adhesion through inhibition of Nox5 signaling pathways and increased RXR/PPAR? and RXR/PPAR? interactions.. Antioxid Redox Signal. 2015 Apr 10;22(11):901-20. doi: 10.1089/ars.2014.5969.
Species: Mouse
Sample type: Conditioned Media -
Bruce A., Ilagan R., Guthrie K., et al. Selected renal cells modulate disease progression in rodent models of chronic kidney disease via NF-?B and TGF-?1 pathways. Regenerative Medicine October 2015 ,Vol. 10, No. 7, Pages 815-839 , DOI 10.2217/rme.15.43 (doi:10.2217/rme.15.43)
Species: Mouse
Sample type: Urine -
Koppe U., Hogner K., Doehn JM., et al. Streptococcus pneumoniae stimulates a STING- and IFN regulatory factor 3-dependent type I IFN production in macrophages, which regulates RANTES production in macrophages, cocultured alveolar epithelial cells, and mouse lungs. J Immunol. 2012 Jan 15;188(2):811-7. doi: 10.4049/jimmunol.1004143.
Species: Mouse
Sample type: Conditioned Media -
Ko G., Linfer D., Jang H., et al. Transcriptional analysis of infiltrating T cells in kidney ischemia-reperfusion injury reveals a pathophysiological role for CCR5. Am J Physiol Renal Physiol. 2012 Mar 15;302(6):F762-73. doi: 10.1152/ajprenal.00335.2011.
Species: Mouse
Sample type: Tissue Lysate
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
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