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Antigen Information
Gene Symbols:
- IGA
- IGE
- IGG2A
- IGHG1
- IGM
Assay Format
Number of Targets Detected:
10
Species Detected:
- Mouse
Compatible Sample Types:
- Cell Culture Supernatants
- Cell Lysates
- Plasma
- Serum
- Tissue Lysates
Design Principle:
- Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
- Semi-Quantitative
Solid Support:
Glass Slide
Product Specifications
Size:
16, 32, or 64 Sample Kit
Product Feature
- One step mouse monoclonal antibody isotyping
- Use only 1-2 µl sample
- The whole experiment can be done within 1 hour
- Sandwich based technology for high specificity and sensitivity
- Low system CV with high reproducibility
- High throughput sample processing
- Qualitative visual inspection or semi-quantitative result
- Processed slides can be stored for years without signal decay
Target Name
IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains
Target Species
Mouse
Application Notes
Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
- Mouse Ig Rapid Isotyping Glass Slide(s)
- Sample Diluent
- 20X Wash Buffer
- Labeled Detection Antibody
- Slide Washer/Dryer
- 96-well Plate
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Mix samples with detection antibodies (in 96-well plate)
- Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.
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Shao W., Zhang C., Liu E., et al. Identification of Liver Epithelial Cell-derived Ig Expression in ? chain-deficient mice. Sci Rep. 2016; 6: 23669. Published online 2016 Mar 29. doi: 10.1038/srep23669
Species: Mouse
Sample type: Tissue Lysate -
Groscurth S., Muller B., Schwan B., et al. Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices. Biomacromolecules. 2012 Oct 8;13(10):3076-86. doi: 10.1021/bm3008499
Species: Mouse
Sample type: Conditioned Media
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
