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Rapid Mouse Ig Isotyping Array 1

RayBio® Rapid Mouse Immunoglobulin Isotyping Array 1. Detects 8 Mouse Ig sub-classes and 2 light chain types. Suitable for serum, plasma, and cell culture supernatants.

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Manual SDS
AAM-ISO-G1-1
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Antigen Information

Gene Symbols:
  • IGA
  • IGE
  • IGG2A
  • IGHG1
  • IGM

Assay Format

Number of Targets Detected:
10
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16, 32, or 64 Sample Kit

Product Feature

  • One step mouse monoclonal antibody isotyping
  • Use only 1-2 µl sample
  • The whole experiment can be done within 1 hour
  • Sandwich based technology for high specificity and sensitivity
  • Low system CV with high reproducibility
  • High throughput sample processing
  • Qualitative visual inspection or semi-quantitative result
  • Processed slides can be stored for years without signal decay

Target Name

IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains

Target Species

Mouse

Application Notes

Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
  • Mouse Ig Rapid Isotyping Glass Slide(s)
  • Sample Diluent
  • 20X Wash Buffer
  • Labeled Detection Antibody
  • Slide Washer/Dryer
  • 96-well Plate
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Mix samples with detection antibodies (in 96-well plate)
  3. Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
  4. Disassemble the glass slide
  5. Scan with a gene microarray laser scanner
  6. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.
Manual SDS
  1. Shao W., Zhang C., Liu E., et al. Identification of Liver Epithelial Cell-derived Ig Expression in ? chain-deficient mice. Sci Rep. 2016; 6: 23669. Published online 2016 Mar 29. doi: 10.1038/srep23669
    Species: Mouse
    Sample type: Tissue Lysate
  2. Groscurth S., Muller B., Schwan B., et al. Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices. Biomacromolecules. 2012 Oct 8;13(10):3076-86. doi: 10.1021/bm3008499
    Species: Mouse
    Sample type: Conditioned Media

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Analysis Tool Bundle Discount

Rapid Mouse Ig Isotyping Array 1
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70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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