Rapid Mouse Ig Isotyping Array 1

RayBio® Rapid Mouse Immunoglobulin Isotyping Array 1. Detects 8 Mouse Ig sub-classes and 2 light chain types. Suitable for serum, plasma, and cell culture supernatants.

AAM-ISO-G1-1
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Antigen Information

Gene Symbols:
  • IGA
  • IGE
  • IGG2A
  • IGHG1
  • IGM

Assay Format

Number of Targets Detected:
10
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16, 32, or 64 Sample Kit

Product Feature

  • One step mouse monoclonal antibody isotyping
  • Use only 1-2 µl sample
  • The whole experiment can be done within 1 hour
  • Sandwich based technology for high specificity and sensitivity
  • Low system CV with high reproducibility
  • High throughput sample processing
  • Qualitative visual inspection or semi-quantitative result
  • Processed slides can be stored for years without signal decay

Target Name

IgA, IgD, IgE, IgM, IgG1, IgG2a, IgG2b, and IgG3 Heavy Chains; Kappa and Lambda Light Chains

Target Species

Mouse

Application Notes

Suggested Application
Isotyping; Monoclonal Antibody Development; Hybridoma Screening and Selection; Biomarker Screening; Confirming Biological Processes
Kit Components
  • Mouse Ig Rapid Isotyping Glass Slide(s)
  • Sample Diluent
  • 20X Wash Buffer
  • Labeled Detection Antibody
  • Slide Washer/Dryer
  • 96-well Plate
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Mix samples with detection antibodies (in 96-well plate)
  3. Incubate with Sample/antibody mix (30 min at 37°C, followed by washes)
  4. Disassemble the glass slide
  5. Scan with a gene microarray laser scanner
  6. Perform densitometry and analysis

Storage/Stability

Upon receipt, all components of the Quantibody Array kit should be stored at -20 °C. At -20 °C the kit will retain complete activity for up to 6 months. The entire kit should be used within 6 months of purchase.

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department ([email protected]).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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