Mouse SDF-1 alpha ELISA

RayBio® Mouse SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-SDF1a-1
+

Antigen Information

Accession Number:
  • P40224
Gene ID:
  • 20315
Gene Symbols:
  • CXCL12
  • SDF1
Protein Name & Synonyms:
Stromal cell-derived factor 1 (SDF-1) (12-O-tetradecanoylphorbol 13-acetate repressed protein 1) (TPAR1) (C-X-C motif chemokine 12) (Pre-B cell growth-stimulating factor) (PBSF) (Thymic lymphoma cell-stimulating factor) (TLSF)
Target Species:
  • Mouse

Assay Format

Detection Range:
3 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

Need more sensitivity? Check out the new IQELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa technology.

Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Burks S., Nguyen B., Tebebi P., et al. Pulsed focused ultrasound pretreatment improves mesenchymal stromal cell efficacy in preventing and rescuing established acute kidney injury in mice. Stem Cells. 2015 Apr;33(4):1241-53. doi: 10.1002/stem.1965.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 Apr;33(4):1173-86. doi: 10.1002/stem.1927
    Species: Mouse
    Sample type: Tissue Lysate
  3. Adipose tissue attracts and protects acute lymphoblastic leukemia cells from chemotherapy
    Species: Mouse
    Sample type: Plasma
  4. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gs?-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample type: Conditioned Media
  5. Lü MJ, Hu CJ, Chen L, Peng X, Chen J, Hu JY, Teng M, Liang GP. miR-27b Represses Migration of Mouse MSCs to Burned Margins and Prolongs Wound Repair through Silencing SDF-1a. PLoS ONE. 8(7): e68972. doi:10.1371/journal.pone.0068972
    Species: Mouse
    Sample type: Conditioned Media
  6. Mallela J, Ravi S, Jean Louis F, et al. NPRA Signaling Regulates Stem Cell Recruitment and Angiogenesis: A Model to Study Linkage Between Inflammation and Tumorigenesis. Stem Cell. 2013 Jul;31(7):1321-1329.
    Species: Mouse
    Sample type: Conditioned Media
  7. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Mallela, Jaya, et al. "Natriuretic peptide receptor A signaling regulates stem cell recruitment and angiogenesis: a model to study linkage between inflammation and tumorigenesis." Stem cells 31.7 (2013): 1321-1329.
    Species: Mouse
    Sample type: Conditioned Media
  9. Hamers A. Vos M., Rassam F., et al. Bone Marrow-Specific Deficiency of Nuclear Receptor Nur77 Enhances Atherosclerosis. Circulation Research. 2012; 110: 428-438. doi: 10.1161/CIRCRESAHA.111.260760
    Species: Mouse
    Sample type: Conditioned Media
  10. Huang C-Y, Shih C-M, Tsao N-W, et al. Dipeptidyl peptidase-4 inhibitor improves neovascularization by increasing circulating endothelial progenitor cells. British Journal of Pharmacology 2012;167(7):1506-1519. doi:10.1111/j.1476-5381.2012.02102.x.
    Species: Mouse
    Sample type: Plasma

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Amazon Gift Card Program

 

Customers Also Bought Customers Also Bought