Mouse SDF-1 alpha ELISA

$398.00
ELM-SDF1a-1

RayBio® Mouse SDF-1 alpha (CXCL12 alpha) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
3 pg/ml

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Detection Range
3 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
Stromal cell-derived factor 1 (SDF-1) (12-O-tetradecanoylphorbol 13-acetate repressed protein 1) (TPAR1) (C-X-C motif chemokine 12) (Pre-B cell growth-stimulating factor) (PBSF) (Thymic lymphoma cell-stimulating factor) (TLSF)
Accession Number
Gene Names
Cxcl12, Sdf1
Gene ID
20315

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CXCL12

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Burks S., Nguyen B., Tebebi P., et al. Pulsed focused ultrasound pretreatment improves mesenchymal stromal cell efficacy in preventing and rescuing established acute kidney injury in mice. Stem Cells. 2015 Apr;33(4):1241-53. doi: 10.1002/stem.1965.
    Species: Mouse
    Sample Type: Tissue Lysate

  2. Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 Apr;33(4):1173-86. doi: 10.1002/stem.1927
    Species: Mouse
    Sample Type: Tissue Lysate

  3. Adipose tissue attracts and protects acute lymphoblastic leukemia cells from chemotherapy
    Species: Mouse
    Sample Type: Conditioned Media

  4. Adipose tissue attracts and protects acute lymphoblastic leukemia cells from chemotherapy
    Species: Mouse
    Sample Type: Plasma

  5. Fulzele K, Krause DS, Panaroni C, et al. Myelopoiesis is regulated by osteocytes through Gsα-dependent signaling. Blood. 2013;121(6):930-939.
    Species: Mouse
    Sample Type: Conditioned Media

  6. Lü MJ, Hu CJ, Chen L, Peng X, Chen J, Hu JY, Teng M, Liang GP. miR-27b Represses Migration of Mouse MSCs to Burned Margins and Prolongs Wound Repair through Silencing SDF-1a. PLoS ONE. 8(7): e68972. doi:10.1371/journal.pone.0068972
    Species: Mouse
    Sample Type: Conditioned Media

  7. Mallela J, Ravi S, Jean Louis F, et al. NPRA Signaling Regulates Stem Cell Recruitment and Angiogenesis: A Model to Study Linkage Between Inflammation and Tumorigenesis. Stem Cell. 2013 Jul;31(7):1321-1329.
    Species: Mouse
    Sample Type: Conditioned Media

  8. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample Type: Conditioned Media

  9. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample Type: Tissue Lysate

  10. Hamers A. Vos M., Rassam F., et al. Bone Marrow-Specific Deficiency of Nuclear Receptor Nur77 Enhances Atherosclerosis. Circulation Research. 2012; 110: 428-438. doi: 10.1161/CIRCRESAHA.111.260760
    Species: Mouse
    Sample Type: Conditioned Media

  11. Huang C-Y, Shih C-M, Tsao N-W, et al. Dipeptidyl peptidase-4 inhibitor improves neovascularization by increasing circulating endothelial progenitor cells. British Journal of Pharmacology 2012;167(7):1506-1519. doi:10.1111/j.1476-5381.2012.02102.x.
    Species: Mouse
    Sample Type: Plasma

  12. H. WANG, Y.-J. et al: Statin Administration Does Not Improve the Mobilization of Very Small Embryonic-Like Stem Cells (VSELs) in Contrast to Resveratrol Treatment in a Murine Model of Acute Myocardial Infarction. Physiol. Res. 61: 543-549, 2012
    Species: Mouse
    Sample Type: Tissue Lysate

  13. Vagima, Y. et al. Early sensing of Yersinia pestis airway infection by bone marrow cells. Front Cell Infect Microbiol. 2012 Nov 26;2:143. doi: 10.3389/fcimb.2012.00143. eCollection 2012.
    Species: Mouse
    Sample Type: Plasma

  14. Lu MH., Li CZ., Hu CJ., Fan YH., et al. microRNA-27b suppresses mouse MSC migration to the liver by targeting SDF-1a in vitro. Biochem Biophys Res Commun. 2012 May 4;421(2):389-95. doi: 10.1016/j.bbrc.2012.04.027
    Species: Mouse
    Sample Type: Tissue Lysate

  15. Li, Meizhang, et al. "Chemokine receptor CXCR4 signaling modulates the growth factor‐induced cell cycle of self‐renewing and multipotent neural progenitor cells." Glia 59.1 (2011): 108-118.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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