Mouse TIMP-1 ELISA

RayBio® Mouse TIMP-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-TIMP1-1
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Antigen Information

Accession Number:
  • P12032
Gene ID:
  • 21857
Gene Symbols:
  • TIMP
  • TIMP-1
  • TIMP1
Protein Name & Synonyms:
Metalloproteinase inhibitor 1 (Collagenase inhibitor 16C8 fibroblast) (Erythroid-potentiating activity) (EPA) (TPA-S1) (TPA-induced protein) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
Target Species:
  • Mouse

Assay Format

Detection Range:
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
10 - 100 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, Leptin (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, MCSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Carroll-Portillo A., Cannon J., Riet J., et al. Mast cells and dendritic cells form synapses that facilitate antigen transfer for T cell activation. J Cell Biol. 2015 Aug 31; 210(5): 851-864. doi: 10.1083/jcb.201412074
    Species: Mouse
    Sample type: Conditioned Media
  2. Song J, Hu D, He C, et al. Novel biomarkers for early prediction of sepsis-induced disseminated intravascular coagulation in a mouse cecal ligation and puncture model. J Inflamm (Lond). 2013;10(1):7 doi:10.1186/1476-9255-10-7
    Species: Mouse
    Sample type: Serum
  3. Song J, Hu D, He C, et al. Novel biomarkers for early prediction of sepsis-induced disseminated intravascular coagulation in a mouse cecal ligation and puncture model. J Inflamm (Lond). 2013;10(1):7.
    Species: Mouse
    Sample type: Serum
  4. Harslund, Jakob, et al. "The role of Serpine-1 and tissue inhibitor of metalloproteinase type-1 in early host responses to Staphylococcus aureus intracutaneous infection of mice." Pathogens and disease 68.3 (2013): 96-104.
    Species: Mouse
    Sample type: Plasma
  5. Kobayashi, Satoshi, et al. "A single dose of lipopolysaccharide into mice with emphysema mimics human chronic obstructive pulmonary disease exacerbation as assessed by micro-computed tomography." American journal of respiratory cell and molecular biology 49.6 (2013): 971-977.
    Species: Mouse
    Sample type: BALF
  6. Spruss A., Kanuri G., Stahl C., et al. Metformin protects against the development of fructose-induced steatosis in mice: role of the intestinal barrier function. Lab Invest. 2012 Jul;92(7):1020-32. doi: 10.1038/labinvest.2012.75
    Species: Mouse
    Sample type: Plasma
  7. Kisarova Y., Korolenko T. Tissue inhibitors of matrix metalloproteinases 1 and 2 and matrix metalloproteinase activity in the serum and lungs of mice with lewis lung carcinoma. Bull Exp Biol Med. 2012 Oct;153(6):874-7.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Harland J, Nielsen OL, Brunner N, Offenberg H. Gender-dependent physiological implications of combines PAI-1 and TIMP-1 gene deficiency characterized in a mouse model. Am J Physiol Regul Integr Comp Physiol. 2007; 293:R1630-R1639.
    Species: Mouse
    Sample type: Plasma
  9. Cuaz-Perolin C, Jguirim I, Larigauderie G, Jlassi A, Furman C, et al. Apolipoprotein E Knockout Mice Over-Expression Human Tissue Inhibitor of Metalloproteinase 1 Are Protected against Aneurysm Formation, but Not Against Atherosclerotic Plaque Development. J Vasc Res. 2006;43:493-501.
    Species: Mouse
    Sample type: Plasma

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.