Mouse TNF-alpha ELISA (For Lysates)

RayBio® Mouse TNF alpha ELISA Kit for cell and tissue lysate samples.


Antigen Information

Accession Number:
  • P06804
Gene ID:
  • 21926
Gene Symbols:
  • TNF
  • TNFSF2
Protein Name & Synonyms:
Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a) [Cleaved into: Tumor necrosis factor, membrane form (N-terminal fragment) (NTF) Intracellular domain 1 (ICD1) Intracellular domain 2 (ICD2) C-domain 1 C-domain 2 Tumor necrosis factor, soluble form]
Target Species:
  • Mouse

Assay Format

Detection Range:
94 pg/ml - 6000 pg/ml
94 pg/ml

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Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1alpha, IL-1beta, IL-2, IL-3, IL-3R beta, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN (OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1alpha, MIP-1gamma, MIP-2, MIP-3 beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • 2X Cell Lysis Buffer
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Zhang S., Wang Y., Li D., et al. Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice. Medicines 2016, 3(3), 16; doi:10.3390/medicines3030016
    Species: Mouse
    Sample type: Tissue Lysate
  2. Trivedi PP., Jena GB., Tikoo KB., Kumar V. Melatonin Modulated Autophagy and Nrf2 Signaling Pathways in Mice With Colitis-Associated Colon Carcinogenesis. Molecular Carcinogenesis. Article first published online: 16 JAN 2015 | DOI: 10.1002/mc.22274
    Species: Mouse
    Sample type: Tissue Lysate
  3. Yu X., Yao JY., He J., Tian JW. Protection of MPTP-induced neuroinflammation and neurodegeneration by rotigotine-loaded microspheres. Life Sci. 2015 Mar 1;124:136-43. doi: 10.1016/j.lfs.2015.01.014
    Species: Mouse
    Sample type: Tissue Lysate
  4. Singla D., Singla R., Abdelli L., Glass C. Fibroblast growth factor-9 enhances M2 macrophage differentiation and attenuates adverse cardiac remodeling in the infarcted diabetic heart. PLoS One. 2015 Mar 13;10(3):e0120739. doi: 10.1371/journal.pone.0120739
    Species: Mouse
    Sample type: Tissue Lysate
  5. Sun J., Wang F., Li H., et al. Neuroprotective Effect of Sodium Butyrate against Cerebral Ischemia/Reperfusion Injury in Mice. Biomed Res Int. 2015;2015:395895. doi: 10.1155/2015/395895.
    Species: Mouse
    Sample type: Tissue Lysate
    Species: Mouse
    Sample type: Tissue Lysate
  7. Wang Y., Li Y., Zhao P., et al. Anti-inflammation Effects of Oxysophoridine on Cerebral Ischemia-Reperfusion Injury in Mice. Inflammation. 2015 Dec;38(6):2259-68. doi: 10.1007/s10753-015-0211-4.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Kabel A., Omar M., Balaha M., Borg H. Effect of metformin and adriamycin on transplantable tumor model. Tissue Cell. 2015 Oct;47(5):498-505. doi: 10.1016/j.tice.2015.07.003.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Zhang X., Zhou Y., Zhang J., et al. IL-35 inhibits acute graft-versus-host disease in a mouse model. Int Immunopharmacol. 2015 Dec;29(2):383-92. doi: 10.1016/j.intimp.2015.10.025
    Species: Mouse
    Sample type: Tissue Lysate
  10. Wu Z., Zhao L., Chen X., et al. Galantamine attenuates amyloid-? deposition and astrocyte activation in APP/PS1 transgenic mice. Exp Gerontol. 2015 Dec;72:244-50. doi: 10.1016/j.exger.2015.10.015.
    Species: Mouse
    Sample type: Tissue Lysate

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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