Mouse VCAM-1 ELISA

RayBio® Mouse VCAM-1 (CD106) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

$402.00  
ELM-VCAM1-1
+

Assay Format

Detection Range:
1.5 pg/ml - 500 pg/ml
Recommended Dilution (serum & plasma):
~200,000 fold
Sensitivity:
1.5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • P29533
Gene ID:
  • 22329
Gene Symbols:
  • VCAM-1
  • VCAM1
Protein Name & Synonyms:
Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (CD antigen CD106)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Schober A., Nazari-Jahantigh M., Wei Y., Bidzhekov K., Gremse F., et al. MicroRNA-126-5p promotes endothelial proliferation and limits atherosclerosis by suppressing Dlk1. Nature Medicine 20, 368-376 (2014)
    Species: Mouse
    Sample type: Tissue Lysate (Mouse Carotid artery Lysate)
  2. Yousef H., Czupalla C., Lee D., et al. Aged blood impairs hippocampal neural precursor activity and activates microglia via brain endothelial cell VCAM1. Nat Med . 2019 Jun;25(6):988-1000. doi: 10.1038/s41591-019-0440-4. Epub 2019 May 13.
    Species: Mouse
    Sample type: Plasma (Aged Immune modeling with Aged blood)
  3. Burks S., Nguyen B., Tebebi P., et al. Pulsed focused ultrasound pretreatment improves mesenchymal stromal cell efficacy in preventing and rescuing established acute kidney injury in mice. Stem Cells. 2015 Apr;33(4):1241-53. doi: 10.1002/stem.1965.
    Species: Mouse
    Sample type: Tissue Lysate (Kidneys from Female C3H mice treated with pulsed focused ultrasound from kidney injured mice)
  4. Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 Apr;33(4):1173-86. doi: 10.1002/stem.1927
    Species: Mouse
    Sample type: Tissue Lysate (Hamstring tissue lysate)
  5. Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats." Biomedicine & Pharmacotherapy 94 (2017): 362-373.
    Species: Rat
    Sample type: Serum (diabetes)
The pre-coated VCAM ELISA kits are highly reproducible and sensitive. Helped me analyze and confirm the VCAM levels that we had detected using the Ray Biotech's AM-CYT-3-8 kit which is a cytokine array kit

W..Keck Center for Transgene Research
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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