Mouse VEGF ELISA

RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Old price: $723.60
$578.88  
You save: $144.72 (20%)
ELM-VEGF-2
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Assay Format

Detection Range:
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Protein Information

Accession Number:
  • Q00731
Gene ID:
  • 22339
Gene Symbols:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Mouse

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Seimetz, Michael, et al. "Inducible NOS inhibition reverses tobacco-smoke-induced emphysema and pulmonary hypertension in mice." Cell 147.2 (2011): 293-305.
    Species: Mouse
    Sample type: Conditioned Media (PASMC, lung endothelial cells (EC), and AECII)
  2. George AL, Rajoria S, Suriano R, Mittleman A, Tiwari RK. Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence. Molecular Cancer. 2012;11:80. doi:10.1186/1476-4598-11-80.
    Species: Mouse
    Sample type: Conditioned Media (TG1-1 cells)
  3. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Tissue Lysate (bladder)
  4. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Conditioned Media (cancer-associated (CAF) and normal fibroblast)
  5. Besmer, Dahlia M., et al. "Pancreatic ductal adenocarcinoma mice lacking mucin 1 have a profound defect in tumor growth and metastasis." Cancer research 71.13 (2011): 4432-4442.
    Species: Mouse
    Sample type: Tissue lysate (tumor tissue)
  6. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27; [Epub ahead of print] doi:10.1038/onc.2013.193.
    Species: Mouse
    Sample type: Cell Lysate (SVEC KS-LT cells, stimulated and controls)
  7. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27; [Epub ahead of print] doi: 10.1038/onc.2013.193.
    Species: Mouse
    Sample type: Conditioned Media (SVEC KS-LT cells, stimulated and controls)
  8. Zhang J., Wu Y., Chen A., Zhao Q. Mesenchymal Stem Cells Promote Cardiac Muscle Repair via Enhanced Neovascularization. Cell Physiol Biochem. 2015;35(3):1219-29. doi: 10.1159/000373945.
    Species: Mouse
    Sample type: Conditioned Media (Mesenchymal Stem cells from Bone marrow, B6 mice)
  9. Degoul F, Borel M, Jacquemot N, et al. In vivo efficacy of melanoma internal radionuclide therapy with a (131) I-labelled melanin-targeting heteroarylcarboxamide molecule. Int J Cancer. 2013;133(5)1042-1053.
    Species: Mouse
    Sample type: Tissue Lysate (melanoma tumor tissue)
  10. Degoul, Françoise, et al. "In vivo efficacy of melanoma internal radionuclide therapy with a 131I?labelled melanin?targeting heteroarylcarboxamide molecule." International journal of cancer 133.5 (2013): 1042-1053.
    Species: Mouse
    Sample type: Tissue Lysate (B16 melanoma tumoural protein extracts)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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