RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • Q00731
Gene ID:
  • 22339
Gene Symbols:
  • VEGF
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
2 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Kondo T., Tsunematsu T., Yamada A., et al. Acceleration of tumor growth due to dysfunction in M1 macrophages and enhanced angiogenesis in an animal model of autoimmune disease. Lab Invest. 2016 Apr;96(4):468-80. doi: 10.1038/labinvest.2015.166.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample type: Tissue Lysate
  3. Enomoto J., Mochizuki N., Ebisawa K., et al. Engineering thick cell sheets by electrochemical desorption of oligopeptides on membrane substrates. Regenerative Therapy Volume 3, March 2016, Pages 24-31
    Species: Mouse
    Sample type: Conditioned Media
  4. Zhang J., Wu Y., Chen A., Zhao Q. Mesenchymal Stem Cells Promote Cardiac Muscle Repair via Enhanced Neovascularization. Cell Physiol Biochem. 2015;35(3):1219-29. doi: 10.1159/000373945.
    Species: Mouse
    Sample type: Conditioned Media
  5. Li Y., Wang W., Huang P., et al. Distinct sustained structural and functional effects of interleukin-33 and interleukin-25 on the airways in a murine asthma surrogate. Immunology. 2015 Aug;145(4):508-18. doi: 10.1111/imm.12465
    Species: Mouse
    Sample type: Tissue Lysate
  6. Pala S., Atilgan R., Ozkan Z., et al. Effect of varying doses of tamoxifen on ovarian histopathology, serum VEGF, and endothelin 1 levels in ovarian hyperstimulation syndrome: an experimental study.
    Species: Mouse
    Sample type: Serum
  7. Patel L., Thaker A. The effects of A2B receptor modulators on vascular endothelial growth factor and nitric oxide axis in chronic cyclosporine nephropathy. J Pharmacol Pharmacother. 2015 Jul-Sep;6(3):147-53. doi: 10.4103/0976-500X.162014.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Abbas OL., et al. Inhibition of the Notch Pathway Promotes Flap Survival by Inducing Functional Neoangiogenesis. Annals of Plastic Surgery August 28, 2014, epub ahead of print. doi: 10.1097/SAP.0000000000000197
    Species: Mouse
    Sample type: Serum
  9. Cai M, Zhou Y, Zhou B, Lou S (2014) Hypoxic Conditioned Medium from Rat Cerebral Cortical Cells Enhances the Proliferation and Differentiation ofNeural Stem Cells Mainly through PI3-K/Akt Pathways. PLoS ONE 9(11): e111938. doi:10.1371/journal.pone.0111938
    Species: Mouse
    Sample type: Conditioned Media
  10. Konac E., et al. Does Pattern Scan Laser (PASCAL) photocoagulation really induce less VEGF expression in murine retina than conventional laser treatment? Gene. In Press, Corrected Proof, Available online 24 July 2014.
    Species: Mouse
    Sample type: Tissue Lysate
The kit was packaged in an organized and clear manner, and it was easy to walk through each step of the procedure. I used the mouse IL-6, VEGF, and TNF-alpha kits for plasma as well as homogenized muscle samples. Each worked well, and I would be happy to use them again.

Houston Methodist Research Institute
Great range of detection for mVEGF. Very straightforward and simple to use. Tear off strips make it convenient to conduct 2-3 assays.

Wonderful product, used for analysis of cell culture supernates and serum samples from animal models. Very clean and easy to use.

Washington University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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