Mouse VEGF ELISA

RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELM-VEGF-1
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Antigen Information

Accession Number:
  • Q00731
Gene ID:
  • 22339
Gene Symbols:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Nasr M., Nafee N., Saad H., Kazem A. Improved antitumor activity and reduced cardiotoxicity of epirubicin using hepatocyte-targeted nanoparticles combined with tocotrienols against hepatocellular carcinoma in mice. European Journal of Pharmaceutics and Biopharmaceutics. Available online 6 May 2014, http://dx.doi.org.proxy.library.emory.edu/10.1016/j.ejpb.2014.04.016 [Epub ahead of print]
    Species: Mouse
    Sample type: Tissue Lysate
  2. Wei, Z., Yu, S. et al. Regulatory Role of the JNK-STAT1/3 Signaling in Neuronal Differentiation of Cultured Mouse Embryonic Stem Cells. Cellular and Molecular Neurobiology 2014. Epub Ahead of Print: DOI: 10.1007/s10571-014-0067-4
    Species: Mouse
    Sample type: Conditioned Media
  3. Hartwich J, Orr WS, Ng CY, et al. HIF-1? activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. J. Pediatr. Surg. 2013 Jan;48(1):39-46.
    Species: Mouse
    Sample type: Serum
  4. Hartwich J., Orr S., Ng C., Spence Y., Morton C., DavidoffA. HIF-1[alpha] activation mediates resistance to anti-angiogenic therapy in neuroblastoma xenografts. Journal of Pediatric Surgery Volume 48, Issue 1 , Pages 39-46, January 2013
    Species: Mouse
    Sample type: Serum
  5. Martin D, Nguyen Q, Molinolo A, Gutkind JS. Accumulation of dephosphorylated 4EBP after mTOR inhibition with rapamycin is sufficient to disrupt paracrine transformation by the KSHV vGPCR oncogene. Oncogene. 2013 May 27; [Epub ahead of print] doi: 10.1038/onc.2013.193.
    Species: Mouse
    Sample type: Conditioned Media
  6. Said N, Frierson HF, Sanchez-Carbayo M, Brekken RA, Theodorescu D. Loss of SPARC in bladder cancer enhances carcinogenesis and progression. J Clin Invest. 2013 February 1; 123(2): 751-766.
    Species: Mouse
    Sample type: Conditioned Media
  7. Saldaña L, Crespo L, Bensiamar F, et al. Mechanical forces regulate stem cell response to surface topography. J Biomed Mater Res A. 2013 Apr 24; [Epub ahead of print] doi:10.1002/jbm.a.34674.
    Species: Human
    Sample type: Conditioned Media
  8. Xiao X, Guo P, Chen Z, et al. Hypoglycemia reduces vascular endothelial growth factor A production by pancreatic beta cells as a regulator of beta cell mass. J Biol Chem. 2013 Mar 22;288(12):8636-8646.
    Species: Mouse
    Sample type: Conditioned Media
  9. Yamamoto Y, Fujita M, Tanaka Y, et al. Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue-Derived Stromal Cells. BioRes Open Access. 2013 May 2;:130502062239007.
    Species: Mouse
    Sample type: Conditioned Media
  10. Degoul, Françoise, et al. "In vivo efficacy of melanoma internal radionuclide therapy with a 131I?labelled melanin?targeting heteroarylcarboxamide molecule." International journal of cancer 133.5 (2013): 1042-1053.
    Species: Mouse
    Sample type: Tissue Lysate
Great range of detection for mVEGF. Very straightforward and simple to use. Tear off strips make it convenient to conduct 2-3 assays.

VCU
Wonderful product, used for analysis of cell culture supernates and serum samples from animal models. Very clean and easy to use.

Washington University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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