Mouse VEGF ELISA

RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-VEGF-1
+

Antigen Information

Accession Number:
  • Q00731
Gene ID:
  • 22339
Gene Symbols:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Yamamoto, Yoritsuna, et al. "Low Oxygen Tension Enhances Proliferation and Maintains Stemness of Adipose Tissue–Derived Stromal Cells." BioResearch open access 2.3 (2013): 199-205.
    Species: Mouse
    Sample type: Conditioned Media
  2. Sumi, Makoto, et al. "Quercetin glucosides promote ischemia-induced angiogenesis, but do not promote tumor growth." Life sciences 93.22 (2013): 814-819.
    Species: Mouse
    Sample type: Serum
  3. Brunner S., Huber B., Weinberger T., et al. Migration of bone marrow-derived cells and improved perfusion after treatment with erythropoietin in a murine model of myocardial infarction. J Cell Mol Med. 2012 Jan;16(1):152-9. doi: 10.1111/j.1582-4934.2011.01286.x.
    Species: Mouse
    Sample type: Serum
  4. George AL, Rajoria S, Suriano R, Mittleman A, Tiwari RK. Hypoxia and estrogen are functionally equivalent in breast cancer-endothelial cell interdependence. Molecular Cancer. 2012;11:80. doi:10.1186/1476-4598-11-80.
    Species: Mouse
    Sample type: Conditioned Media
  5. Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.
    Species: Mouse
    Sample type: Conditioned Media
  6. Altman M., Nguyen D., Patel S., et al. Regulator of G-Protein Signaling 5 Reduces HeyA8 Ovarian Cancer Cell Proliferation and Extends Survival in a Murine Tumor Model. Biochem Res Int. 2012;2012:518437. doi: 10.1155/2012/518437
    Species: Mouse
    Sample type: Serum
  7. Jones L., Antonelli J., Masko E., et al. Exercise modulation of the host-tumor interaction in an orthotopic model of murine prostate cancer. J Appl Physiol (1985). 2012 Jul;113(2):263-72. doi: 10.1152/japplphysiol.01575.2011.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Sawai Y., Murata H., Koto K., et al. Effects of low-intensity pulsed ultrasound on osteosarcoma and cancer cells. Oncol Rep. 2012 Aug;28(2):481-6. doi: 10.3892/or.2012.1816.
    Species: Mouse
    Sample type: Conditioned Media
  9. Clarke D., Al Ahmad A., Lee B., et al. Perlecan Domain V induces VEGf secretion in brain endothelial cells through integrin ?5?1 and ERK-dependent signaling pathways. PLoS One. 2012;7(9):e45257. doi: 10.1371/journal.pone.0045257
    Species: Mouse
    Sample type: Conditioned Media
  10. Chen, Wenxing, et al. "Beta-elemene inhibits melanoma growth and metastasis via suppressing vascular endothelial growth factor-mediated angiogenesis." Cancer chemotherapy and pharmacology 67.4 (2011): 799-808.
    Species: Mouse
    Sample type: Tissue lysate
Great range of detection for mVEGF. Very straightforward and simple to use. Tear off strips make it convenient to conduct 2-3 assays.

VCU
Wonderful product, used for analysis of cell culture supernates and serum samples from animal models. Very clean and easy to use.

Washington University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.