Mouse VEGF ELISA

RayBio® Mouse VEGF-A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELM-VEGF-1
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Antigen Information

Accession Number:
  • Q00731
Gene ID:
  • 22339
Gene Symbols:
  • VEGF
  • VEGFA
Protein Name & Synonyms:
Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF)
Target Species:
  • Mouse

Assay Format

Detection Range:
2 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
3 fold
Sensitivity:
2 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, Tpo.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Weir, Lynda, et al. "Hypoxia-mediated control of HIF/ARNT machinery in epidermal keratinocytes." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1813.1 (2011): 60-72.
    Species: Mouse
    Sample type: Cell Lysate
  2. Shields, Kristin M., et al. "Mitogen-activated protein kinase phosphatase-1 is a key regulator of hypoxia-induced vascular endothelial growth factor expression and vessel density in lung." The American journal of pathology 178.1 (2011): 98-109.
    Species: Mouse
    Sample type: Cell Lysate
  3. Gao, Feng, et al. "L-5F, an apolipoprotein AI mimetic, inhibits tumor angiogenesis by suppressing VEGF/basic FGF signaling pathways." Integrative Biology 3.4 (2011): 479-489.
    Species: Mouse
    Sample type: Conditioned Media
  4. Yoshida, Atsuhiro, et al. "Adipose tissue?derived stromal cells protect hair cells from aminoglycoside." The Laryngoscope 121.6 (2011): 1281-1286.
    Species: Mouse
    Sample type: Conditioned Media
  5. Besmer, Dahlia M., et al. "Pancreatic ductal adenocarcinoma mice lacking mucin 1 have a profound defect in tumor growth and metastasis." Cancer research 71.13 (2011): 4432-4442.
    Species: Mouse
    Sample type: Tissue lysate
  6. Seimetz, Michael, et al. "Inducible NOS inhibition reverses tobacco-smoke-induced emphysema and pulmonary hypertension in mice." Cell 147.2 (2011): 293-305.
    Species: Mouse
    Sample type: Conditioned Media
  7. Bhuvaneswari R, Gan Y, Yee K, Soo K,Olivo M. Effect of hypericin-mediated photodynamic therapy on the expression of vascular endothelial growth factor in human nasopharyngeal carcinoma. Int J Mol Med. 2007;20:421-428.
    Species: Mouse
    Sample type: Tissue Lysate
Great range of detection for mVEGF. Very straightforward and simple to use. Tear off strips make it convenient to conduct 2-3 assays.

VCU
Wonderful product, used for analysis of cell culture supernates and serum samples from animal models. Very clean and easy to use.

Washington University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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