Purity determined by:
> 90% pure (SDS-PAGE). Virus was grown on monolayer of HeLa cells. When the cultures showed pronounced c.p.e. virus containing medium was purified from cell fragments by low speed centrifugation (4,000 RPM 40 min). Virus was pelleted by high speed centrifugation at 18,000 RPM for 2.5 hours in the SW27 rotor for 20 min at 4°,C, then the supernatant was collected.
Purified, Liquid Exp. Date: N/A
0.05 M Tris-HCl, pH 8.0 containing 0.1 M Sodium Chloride, 5 mM EDTA.
1.3 mg/mL (Lowry)
0.1% Sodium Azide and 0.005% Thimerosal.
Specific methodologies have not been tested using this product.
Thimerosal and beta propiolactone.
Store at -20°C. Avoid multiple freeze/thaw cycles.