Biological activity:
20-22 U/mg protein. 1 unit hydrolyzes one μmole per minute of MeO-Suc-Ala-Ala-Pro-Val-pNA at 25ºC, in 100mM Tris-Hcl, pH 7.5 with 500mM NaCl.
Format:
Purified protein from human neutrophils - liquid
Formulation:
50mM sodium acetate, pH 5.5
150mM sodium chloride
Concentration (lot specific):
∼1.0 mg/ml
Preservative:
None present.
Recommended Applications:
ELISA
Protein Name & Synonyms:
ELASTASE,
Molecular Weight (kDa):
28.5 kDa
Human Elastase is a 28.5kDa serine protease that has been purified from human neutrophils. Elastase is also present in the pancreas, leucocytes and serum.
Storage/Stability
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for12 months from date of dispatch.