Protein Name & Synonyms:
LOW DENSITY LIPOPROTEIN,
Purity determined by:
Purified LDL is acetylated and then labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate). The resultant product is exhaustively dialyzed against 0.15 M NaCl, 0.05 M Tris, pH 7.4, 0.3 mM EDTA, sterilized by filtration and then aseptically packaged.
Purified acetylated LDL from human serum, Dil labelled - liquid
Total protein concentration ~0.2 mg/ml
Functional Assays, Flow Cytometry, Immunofluorescence 3% formaldehyde in PBS is required for fixation. Methanol or Acetone can not be used as DS-01-0290 is soluble in organic solvents.
Many lots have been evaluated for the specific labelling of bovine aortic endothelium, HUVECS, murine macrophages and the current lot was evaluated on P-388D cells.
Store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is stable when handled aseptically and stored at 4°C. Please see label for expiry date.