Native Human LOW DENSITY LIPOPROTEIN (ACETYLATED):DIL

DS-01-0290
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Source

Species:
Human
Protein Name & Synonyms:
LOW DENSITY LIPOPROTEIN,

Preparation

Purity determined by:
Purified LDL is acetylated and then labelled with the fluorescent probe Dil (1,1'-dioctadecyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate). The resultant product is exhaustively dialyzed against 0.15 M NaCl, 0.05 M Tris, pH 7.4, 0.3 mM EDTA, sterilized by filtration and then aseptically packaged.

Protein Specifications

Format:
Purified acetylated LDL from human serum, Dil labelled - liquid
Concentration:
Total protein concentration ~0.2 mg/ml
Preservative:
None present.
Recommended Applications:
Functional Assays, Flow Cytometry, Immunofluorescence 3% formaldehyde in PBS is required for fixation. Methanol or Acetone can not be used as DS-01-0290 is soluble in organic solvents.

Description

Many lots have been evaluated for the specific labelling of bovine aortic endothelium, HUVECS, murine macrophages and the current lot was evaluated on P-388D cells.

Specifications

Size
200 ug

Stability & Storage

Storage/Stability
Store at +4°C. DO NOT FREEZE. This product should be stored undiluted. This product is stable when handled aseptically and stored at 4°C. Please see label for expiry date.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.