Concentration (lot specific):
4.7 mg/mL (Bio-Rad Dye Binding Microassay) (minimum concentration 1 mg/mL). Titer is controlled lot-to-lot by ELISA IgG reactivity.
Purity determined by:
Mouse L cells are infected with C. trachomatis elementary bodies. Optimally infected cells are harvested and disrupted by sonication in PBS. Cellular debris is removed using centrifugation.
Should be sonicated immediately prior to use to ensure that the preparation is uniform. Used for both IgG and IgM detection in assays which include EIA with polystyrene and latex solid phases and M capture formats. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Mouse L Cells
Inactivated using gamma radiation. Effective primarily by damaging chlamydial genetic material.
Store at < -70°C. Avoid multiple freeze/thaw cycles.