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Obesity Arrays

Obesity and the diseases arising from it (diabetes, cardiovascular disease and cancer) constitute a major health challenge to the industrialized world. In the past decade, it has become apparent that adipose tissue is not an inert fat-storage depot, but a major endocrine organ which secretes numerous soluble factors and regulates many processes related to glucose homeostasis, lipid and protein metabolism, hunger/satiety, inflammatory and immune responses, and vascular function. Collectively, these factors are referred to as adipokines. Some are mainly produced by adipose tissue (e.g., leptin and resistin), while others are classical cytokines such as TNF-α, IL-6, MCP-1, and IL-1, which are also synthesized in other tissues. Understanding adipokine-mediated molecular signaling is critical to understanding and treating the myriad of diseases resulting from obesity.

RayBiotech offers 5 different Obesity Antibody Arrays for detecting up to 62 Adipokines. See the below product listings for more information.

Human Obesity Arrays

Human Obesity Array Q1

Human Obesity Array Q1

(QAH-ADI-1)

Quantibody Arrays - Quantitative, Sandwich-Based, Glass Slide Antibody Arrays

Detects 10 Human Adipokines:

IGF-1, IL-1 beta (IL-1 F2), IL-6, IL-8 (CXCL8), Insulin, Leptin, MCP-1 (CCL2), PAI-1, Resistin, TNF alpha

PDFManualOverviewQuantibody Overview

Human Obesity Array Q3

Human Obesity Array Q3

(QAH-ADI-3)

Quantibody Arrays - Quantitative, Sandwich-Based, Glass Slide Antibody Arrays

Detects 40 Human Adipokines:

Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, ANGPTL4, BDNF, Chemerin, CRP (C-Reactive Protein), Growth Hormone, IFN-gamma, IGFBP-1, IGFBP-2, IGF-1, IL-10, IL-12 p40, IL-12 p70, IL-1 beta (IL-1 F2), IL-1 ra (IL-1 F3), IL-6, IL-8 (CXCL8), Insulin, Leptin, Lipocalin-2 (NGAL), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), PAI-1, PDGF-BB, Pepsinogen 1, Pepsinogen 2, Procalcitonin, Prolactin, RANTES (CCL5), RBP4, Resistin, SAA (Serum Amyloid A), TGF beta 1, Thrombospondin 1, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TNF alpha, VEGF-A

PDFManualOverviewQuantibody Overview

L-Series Human Obesity Antibody Array 182

Human L182 Array, Membrane

(AAH-BLM-ADI-1)

L-Series Arrays - Semi-quantitative, label-Based, high density antibody arrays

Detects 182 Human Adipokines:

ACE / CD43, ACE-2, ACTH, ADFP, Adiponectin, Adipsin (Factor D), AgRP , AMPKa1 , Amylin, Angiopoietin 1, Angiopoietin 2, Angiotensinogen / Angiotensin II, Ang-like Factor, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4 , Apelin Receptor , ApoB, ApoE, Axl, BDNF, bFGF, BMP-2 , BMP-3, BMP-3b / GDF-10, BMP-4 , BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA / ALK-3, BMPR-IB / ALK-6, BMPR-II, b-NGF, C3a des Arg, CART , CD137 (4-1BB), CD36, Clusterin, CNTF, C-peptide, CRP , Cystatin C, Dtk, EGF, EGFR, ENA-78, Endophin Beta, Epiregulin, E-selectin, ET-1 (Endothelin), FABP4, FAM3B, FAS / Apo-1, FGF-10, FGF-6, FSH , Galectin -1, Growth Hormone (Growth Hormone), Ghrelin, GITR, GITR Ligand, GLP-1 , Glucagon, Glut1, Glut2, Glut3, Glut5, Glutathione peroxidase 1, Glutathione peroxidase 3, GRO alpha, HCC4, HGF, HSD-1, ICAM1, IFN-gamma, IGF-1, ...View all 182 human adipokine proteins

PDFManualOverviewL-Series Overview

Human Obesity Array C1

Human Obesity Array C1

(AAH-ADI-1)

C-Series Arrays - Semi-Quantitative, Sandwich-Based, Membrane Antibody Arrays

Detects 62 Human Adipokines:

4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, Angiopoietin-1, Angiopoietin-2, ANGPTL4, CRP (C-Reactive Protein), ENA-78 (CXCL5), Fas (TNFRSF6/Apo-1), FGF-6, Growth Hormone, HCC-4 (CCL16), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p70, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-6, IL-6 R, IL-8 (CXCL8), Insulin, IP-10 (CXCL10), Leptin R, Leptin, LIF, Lymphotactin (XCL1), MCP-1 (CCL2), MCP-3 (MARC/CCL7), M-CSF, MIF, MIP-1 beta (CCL4), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), Oncostatin M, PAI-1, PARC (CCL18), PDGF-AA, PDGF-AB, PDGF-BB, RANTES (CCL5), Resistin, SAA (Serum Amyloid A), SDF-1 alpha (CXCL12 alpha), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TECK (CCL25), TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR

PDFManualOverviewC-Series Overview

Human Obesity Array G1

Human Obesity Array G1

(AAH-ADI-G1)

G-Series Arrays - Semi-Quantitative, Sandwich-Based, Glass Slide Antibody Arrays

Detects 62 Human Adipokines:

4-1BB (TNFRSF9/CD137), ACE-2, Adiponectin (ACRP30), Adipsin (Complement Factor D), AgRP, Angiopoietin-1, Angiopoietin-2, ANGPTL4, CRP (C-Reactive Protein), ENA-78 (CXCL5), Fas (TNFRSF6/Apo-1), FGF-6, Growth Hormone, HCC-4 (CCL16), IFN-gamma, IGFBP-1, IGFBP-2, IGFBP-3, IGF-1, IGF-1 R, IL-1 R4 (ST2), IL-1 R1, IL-10, IL-11, IL-12 p70, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-6, IL-6 R, IL-8 (CXCL8), Insulin, IP-10 (CXCL10), Leptin R, Leptin, LIF, Lymphotactin (XCL1), MCP-1 (CCL2), MCP-3 (MARC/CCL7), M-CSF, MIF, MIP-1 beta (CCL4), MSP alpha/beta, Osteoprotegerin (TNFRSF11B), Oncostatin M, PAI-1, PARC (CCL18), PDGF-AA, PDGF-AB, PDGF-BB, RANTES (CCL5), Resistin, SAA (Serum Amyloid A), SDF-1 alpha (CXCL12 alpha), TNF RII (TNFRSF1B), TNF RI (TNFRSF1A), TECK (CCL25), TGF beta 1, TIMP-1, TIMP-2, TNF alpha, VEGF-A, XEDAR

PDFManualOverviewG-Series Overview

Product Features

  • Quantitative or Semi-Quantitative level of up to 62 Adipokines are detected simultaneously
  • No need to perform individual immunoprecipitations or Western Blot
  • Reliable results with wide range of cell lines
  • High specificity, sensitivity and reproducibility
  • Membrane formats - No special equipment required
  • Glass slide formats - Fluorescent laser scanner is needed*
  • Many choices: chemiluminescence or fluorescence detection system; Membrane or glass chip

*Click here for a list of compatible laser scanners. Raybiotech now offers free glass slide scanning and data extraction services (click to learn more!).

How It Works

The principle of how an antibody array works

Publications Citing RayBiotech Products

Obese patients with elevated expression transcription factor E2F1 in their visceral adipose tissue have a high cardiometabolic risk. In this study, Maixner et al. used unbiased transcriptomic analysis of E2F1high versus E2F1low human VAT to identify the E2F1-mediated pathways that result in metabolic dysfunction. TRAIL, TL1A, and their receptors were upregulated in E2F1high samples with tissue-specific enrichment patterns. Two independent human cohorts confirmed the associations between high TL1A and TRAIL expression and increased leptin and IL6 serum concentrations, diabetes status, and hVAT-macrophage lipid content. Their data link high VAT E2F1 expression with adipose tissue dysfunction.

Maixner N, et al. A TRAIL-TL1A Paracrine Network Involving Adipocytes, Macrophages, and Lymphocytes Induces Adipose Tissue Dysfunction Downstream of E2F1 in Human Obesity. Diabetes (2020). [view publication]
RayBiotech Products: IQELISA (cat no. IQH-TRAIL)
Species: Human
Sample Type: Conditioned medium

Atherogenesis is caused in part by LOX-1, which is a membrane glycoprotein that regulates the formation of foam cells from macrophages and their accumulation in lipid laden regions. Dai et al.'s study show that the micro RNA, miR-98, targets LOX-1. By treating cultured macrophages with miR-98, LOX-1 expression and subsequent foam cell formation was inhibited. In mice, treatment with a double-strand miRNA mimic, agomiR-98, significantly reduced lipid accumulation in the aortas.

Dai Y, et al. MicroRNA-98 regulates foam cell formation and lipid accumulation through repression of LOX-1. Redox Biol (2018). [view publication]
RayBiotech Products: Sandwich ELISA (cat no. ELM-LOX1)
Species: Mouse
Sample Type: Plasma

Extracellular vesicles (EVs) offer an alternative approach to cell-based regenerative medicine. In this study, mice were treated with EVs isolated from human adipose-derived stem cells (HASCs) during white and beige adipogenic differentiation (D-EV and BD-EV, respectively). miRNAs in the BD-EVs altered diet-induced obesity, thus improving diet-related hepatic steatosis and glucose tolerance. These results reveal that EVs can promote cell reprogramming.

Jung YJ, et al. Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes. Sci Adv. (2020). [view publication]
RayBiotech Products: G-Series (cat no. AAH-ADI-G1)
Species: Human
Sample Type: Tissue lysate

Inflammasomes are major mediators of innate immunity. In the context of obesity, the NLRP3 inflammasome plays an important role in adipose tissue (AT)-induced inflammation and associated comorbidities. Here, patients with nonalcoholic steatohepatitis (NASH) and type 2 diabetes (T2D) had significantly increased NLRP3 mRNA levels (p < 0.05) and its proinflammatory mediator IL1B (p < 0.01) in the liver compared to controls. Additional experiments revealed that inhibiting NLRP3 in human visceral adipocytes significantly blocked (p < 0.01) LPS-induced inflammation. These data help understand the metabolic alterations - and expression of inflammatory mediators - in obesity.

Unamuno X, et al. NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling. Cell Mol Immunol (2019). [view publication]
RayBiotech Products: ELISA (cat no. ELH-IL1b)
Species: Human
Sample Type: Conditioned medium

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