Commercially available plates for DNA binding often require chemical modifications to the DNA, such as amines or thiols. The Oligo Binding Buffer is used to bind unmodified DNA onto polystyrene plastics. Unlike other products available, using this buffer enables the researcher to bind unmodified DNA to plastics.
DNA can be bound to a variety of plastics, such as bead or microplates, to facilitate complement DNA capture, hybridization assays, or any other application which requires DNA to be immobilized on a surface.
Because DNA can vary in sequence and weight, care should be taken to optimize binding for each application. For most applications 2µg/ml of DNA is sufficient, however more DNA can be used if necessary. Prepare solutions by mixing 1 volume of DNA with 1 volume of DNA binding buffer. Immediately after mixing add the DNA solution to the plastic that the DNA is to be bound to. For best results incubate the reaction overnight at 4ᵒC with rocking, however binding can be observed after as few as 3 hours.
After incubation remove the supernatant and wash with an appropriate buffer such as PBS. Detergents such as Tween can be included in washing if necessary, although SDS should be avoided if possible. While polystyrene plastics are recommended, If DNA is bound to a polypropylene substrate avoid using detergents as this can disrupt the DNA-plastic interaction.
Measurement of DNA bound to wells of a polystyrene plate at a variety of concentrations.
Buffer should be stored at room temperature, as storage at low temperatures can result in precipitate formation. If any precipitate is observed simply apply gentle heating to 37ᵒC and mixing to dissolve any precipitate.
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.