PD-1 Epitope Mapping Peptide Array

The PD-1 (programmed cell death protein 1/CD279) Epitope Mapping Peptide Array is a semi-quantitative array displaying 254 synthesized peptides representing the full-length PD-1. It is suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc. in serum, plasma, and many other liquid sample types.

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PAH-PD1-G1

Assay Format

Species Detected:
  • Human
Compatible Sample Types:
  • Ascites
  • DNA Aptamers
  • Hybridoma cell culture medium
  • Other fluids
  • Plasma
  • Purified antibodies
  • Purified protein
  • Serum
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Glass Slide
Antibody Type:
Human IgG, mouse IgG, other isotypes (IgA, IgM, IgE, etc.) derived from most animals, upon request.

Product Specifications

Size:
1, 2, or 4 Slide Kit

Protein Information

Accession Number:
  • Q15116
Gene Symbols:
  • PDCD1
Protein Name & Synonyms:
PD-1 / programmed cell death protein 1 / CD279
Target Species:
Human
Synthesized Protein Sequence:
256 amino acids (Phe24-Leu288, without N-terminal signal peptide)
FLDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSNQTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCGAISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQTLVVGVVGGLLGSLVLLVWVLAVICSRAARGTIGARRTGQPLKEDPSAVPVFSVDYGELDFQWREKTPEPPVPCVPEQTEYATIVFPSGMGTSSPARRGSADGPRSAQPLRPEDGHCSWPL

Peptide Information

Number of Peptides:
254
Peptide Length:
12-mer
Peptide Offset:
1-mer
Peptide Purity:
HPLC grade, >95% purity
Array Printing Replicate:
Triplicate

Peptide Sequence

1. FLDSPDRPWNPP 2. LDSPDRPWNPPT 3. DSPDRPWNPPTF 4. SPDRPWNPPTFS
5. PDRPWNPPTFSP 6. DRPWNPPTFSPA 7. RPWNPPTFSPAL 8. PWNPPTFSPALL
9. WNPPTFSPALLV 10. NPPTFSPALLVV 11. PPTFSPALLVVT 12. PTFSPALLVVTE
13. TFSPALLVVTEG 14. FSPALLVVTEGD 15. SPALLVVTEGDN 16. PALLVVTEGDNA
17. ALLVVTEGDNAT 18. LLVVTEGDNATF 19. LVVTEGDNATFT 20. VVTEGDNATFTC
21. VTEGDNATFTCS 22. TEGDNATFTCSF 23. EGDNATFTCSFS 24. GDNATFTCSFSN
25. DNATFTCSFSNT 26. NATFTCSFSNTS 27. ATFTCSFSNTSE 28. TFTCSFSNTSES
29. FTCSFSNTSESF 30. TCSFSNTSESFV 31. CSFSNTSESFVL 32. SFSNTSESFVLN
33. FSNTSESFVLNW 34. SNTSESFVLNWY 35. NTSESFVLNWYR 36. TSESFVLNWYRM
37. SESFVLNWYRMS 38. ESFVLNWYRMSP 39. SFVLNWYRMSPS 40. FVLNWYRMSPSN
41. VLNWYRMSPSNQ 42. LNWYRMSPSNQT 43. NWYRMSPSNQTD 44. WYRMSPSNQTDK
45. YRMSPSNQTDKL 46. RMSPSNQTDKLA 47. MSPSNQTDKLAA 48. SPSNQTDKLAAF
49. PSNQTDKLAAFP 50. SNQTDKLAAFPE 51. NQTDKLAAFPED 52. QTDKLAAFPEDR
53. TDKLAAFPEDRS 54. DKLAAFPEDRSQ 55. KLAAFPEDRSQP 56. LAAFPEDRSQPG
57. AAFPEDRSQPGQ 58. AFPEDRSQPGQD 59. FPEDRSQPGQDC 60. PEDRSQPGQDCR
61. EDRSQPGQDCRF 62. DRSQPGQDCRFR 63. RSQPGQDCRFRV 64. SQPGQDCRFRVT
65. QPGQDCRFRVTQ 66. PGQDCRFRVTQL 67. GQDCRFRVTQLP 68. QDCRFRVTQLPN
69. DCRFRVTQLPNG 70. CRFRVTQLPNGR 71. RFRVTQLPNGRD 72. FRVTQLPNGRDF
73. RVTQLPNGRDFH 74. VTQLPNGRDFHM 75. TQLPNGRDFHMS 76. QLPNGRDFHMSV
77. LPNGRDFHMSVV 78. PNGRDFHMSVVR 79. NGRDFHMSVVRA 80. GRDFHMSVVRAR
81. RDFHMSVVRARR 82. DFHMSVVRARRN 83. FHMSVVRARRND 84. HMSVVRARRNDS
85. MSVVRARRNDSG 86. SVVRARRNDSGT 87. VVRARRNDSGTY 88. VRARRNDSGTYL
89. RARRNDSGTYLC 90. ARRNDSGTYLCG 91. RRNDSGTYLCGA 92. RNDSGTYLCGAI
93. NDSGTYLCGAIS 94. DSGTYLCGAISL 95. SGTYLCGAISLA 96. GTYLCGAISLAP
97. TYLCGAISLAPK 98. YLCGAISLAPKA 99. LCGAISLAPKAQ 100. CGAISLAPKAQI
101. GAISLAPKAQIK 102. AISLAPKAQIKE 103. ISLAPKAQIKES 104. SLAPKAQIKESL
105. LAPKAQIKESLR 106. APKAQIKESLRA 107. PKAQIKESLRAE 108. KAQIKESLRAEL
109. AQIKESLRAELR 110. QIKESLRAELRV 111. IKESLRAELRVT 112. KESLRAELRVTE
113. ESLRAELRVTER 114. SLRAELRVTERR 115. LRAELRVTERRA 116. RAELRVTERRAE
117. AELRVTERRAEV 118. ELRVTERRAEVP 119. LRVTERRAEVPT 120. RVTERRAEVPTA
121. VTERRAEVPTAH 122. TERRAEVPTAHP 123. ERRAEVPTAHPS 124. RRAEVPTAHPSP
125. RAEVPTAHPSPS 126. AEVPTAHPSPSP 127. EVPTAHPSPSPR 128. VPTAHPSPSPRP
129. PTAHPSPSPRPA 130. TAHPSPSPRPAG 131. AHPSPSPRPAGQ 132. HPSPSPRPAGQF
133. PSPSPRPAGQFQ 134. SPSPRPAGQFQT 135. PSPRPAGQFQTL 136. SPRPAGQFQTLV
137. PRPAGQFQTLVV 138. RPAGQFQTLVVG 139. PAGQFQTLVVGV 140. AGQFQTLVVGVV
141. GQFQTLVVGVVG 142. QFQTLVVGVVGG 143. FQTLVVGVVGGL 144. QTLVVGVVGGLL
145. TLVVGVVGGLLG 146. LVVGVVGGLLGS 147. VVGVVGGLLGSL 148. VGVVGGLLGSLV
149. GVVGGLLGSLVL 150. VVGGLLGSLVLL 151. VGGLLGSLVLLV 152. GGLLGSLVLLVW
153. GLLGSLVLLVWV 154. LLGSLVLLVWVL 155. LGSLVLLVWVLA 156. GSLVLLVWVLAV
157. SLVLLVWVLAVI 158. LVLLVWVLAVIC 159. VLLVWVLAVICS 160. LLVWVLAVICSR
161. LVWVLAVICSRA 162. VWVLAVICSRAA 163. WVLAVICSRAAR 164. VLAVICSRAARG
165. LAVICSRAARGT 166. AVICSRAARGTI 167. VICSRAARGTIG 168. ICSRAARGTIGA
169. CSRAARGTIGAR 170. SRAARGTIGARR 171. RAARGTIGARRT 172. AARGTIGARRTG
173. ARGTIGARRTGQ 174. RGTIGARRTGQP 175. GTIGARRTGQPL 176. TIGARRTGQPLK
177. IGARRTGQPLKE 178. GARRTGQPLKED 179. ARRTGQPLKEDP 180. RRTGQPLKEDPS
181. RTGQPLKEDPSA 182. TGQPLKEDPSAV 183. GQPLKEDPSAVP 184. QPLKEDPSAVPV
185. PLKEDPSAVPVF 186. LKEDPSAVPVFS 187. KEDPSAVPVFSV 188. EDPSAVPVFSVD
189. DPSAVPVFSVDY 190. PSAVPVFSVDYG 191. SAVPVFSVDYGE 192. AVPVFSVDYGEL
193. VPVFSVDYGELD 194. PVFSVDYGELDF 195. VFSVDYGELDFQ 196. FSVDYGELDFQW
197. SVDYGELDFQWR 198. VDYGELDFQWRE 199. DYGELDFQWREK 200. YGELDFQWREKT
201. GELDFQWREKTP 202. ELDFQWREKTPE 203. LDFQWREKTPEP 204. DFQWREKTPEPP
205. FQWREKTPEPPV 206. QWREKTPEPPVP 207. WREKTPEPPVPC 208. REKTPEPPVPCV
209. EKTPEPPVPCVP 210. KTPEPPVPCVPE 211. TPEPPVPCVPEQ 212. PEPPVPCVPEQT
213. EPPVPCVPEQTE 214. PPVPCVPEQTEY 215. PVPCVPEQTEYA 216. VPCVPEQTEYAT
217. PCVPEQTEYATI 218. CVPEQTEYATIV 219. VPEQTEYATIVF 220. PEQTEYATIVFP
221. EQTEYATIVFPS 222. QTEYATIVFPSG 223. TEYATIVFPSGM 224. EYATIVFPSGMG
225. YATIVFPSGMGT 226. ATIVFPSGMGTS 227. TIVFPSGMGTSS 228. IVFPSGMGTSSP
229. VFPSGMGTSSPA 230. FPSGMGTSSPAR 231. PSGMGTSSPARR 232. SGMGTSSPARRG
233. GMGTSSPARRGS 234. MGTSSPARRGSA 235. GTSSPARRGSAD 236. TSSPARRGSADG
237. SSPARRGSADGP 238. SPARRGSADGPR 239. PARRGSADGPRS 240. ARRGSADGPRSA
241. RRGSADGPRSAQ 242. RGSADGPRSAQP 243. GSADGPRSAQPL 244. SADGPRSAQPLR
245. ADGPRSAQPLRP 246. DGPRSAQPLRPE 247. GPRSAQPLRPED 248. PRSAQPLRPEDG
249. RSAQPLRPEDGH 250. SAQPLRPEDGHC 251. AQPLRPEDGHCS 252. QPLRPEDGHCSW
253. PLRPEDGHCSWP 254. LRPEDGHCSWPL

Application Notes

  • Identify high antigenicity epitopes for antibody production & vaccine development
  • Locate (auto)antigen epitopes of (auto)antibodies
  • Define the best antibody clone against the protein-of-interest
  • Perform epitope mutant analysis
  • Map B cell epitopes

Service Features

  • Small serum sample volume required (2 µl)
  • High density (simultaneously analyze hundreds of overlapping peptides)
  • High sensitivity
  • Large dynamic range
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use

Kit Components

  • RayBio® human PD-1 protein peptide array
  • Blocking Buffer
  • Wash Buffer I
  • Wash Buffer II
  • Biotin-conjugated secondary antibody
  • Cy3 equivalent dye conjugated streptavidin
  • Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)

Other Materials Required

  • Distilled water
  • Small plastic boxes or containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Storage/Stability

Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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