Rabbit Anti-Human Phospho-AKT1(S473)

Rabbit Anti-Human Phospho-AKT1(S473) Antibody, 400 ul

$443.00  
102-15425
+

Antigen Information

Accession Number:
  • P31749
Gene ID:
  • 207
Gene Symbols:
  • AKT1
Protein Name & Synonyms:
Phospho-AKT1(S473)
Target Species:
  • Human

Antibody Specifications

Concentration:
0.500 mg/ml
Preparation:
This Phospho-AKT1-pS473 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S473 of human AKT1.
Species Reactivity:
Human
Clonality:
Polyclonal

Introduction

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Specificity

Other Names
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha
NCBI Accession #
NP_001014431.1;NP_001014432.1;NP_005154.2

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55686
Recommended Dilutions
DB: 1:500; WB: 1:1000
Dot blot analysis of anti-AKT1-pS473 Phospho-specific Pab (Cat.102-15425) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Western blot analysis of extracts from NIH-3T3 cells,untreated or treated with PDGF,using Phospho-Akt(Ser473)(left) or Akt antibody(right).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.