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A Guide to RayBiotech Phosphorylation Assays

Protein phosphorylation plays a prominent role in cell signaling, development and growth. RayBiotech provides a line of phosphorylation immunoassays for monitoring pathway activation, offering a rapid and convenient alternative to standard immunoprecipitations and Western Blots.

AKT Pathway Phosphorylation Array

Assay PrincipleSandwich-based array
Sample TypesLysates
Targets DetectedPhosphorylated human/mouse proteins involved in AKT pathway
ApplicationDetection of phosphorylation signatures; pathway analysis
Solid Support DetectionMembrane (chemiluminescence)

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Apoptosis Signaling Array

Assay PrincipleSandwich-based array
Sample TypesLysates
Targets DetectedPhosphorylated and cleaved human apoptotic factors
ApplicationsDetection of apoptosis signaling; pathway analysis
Solid Support DetectionMembrane (chemiluminescence)

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RTK Phosphorylation Array

Assay PrincipleSandwich-based array
Sample TypesLysates
Targets DetectedPhosphorylated tyrosine kinase receptors
ApplicationsDetection of phosphorylation signatures; pathway analysis
Solid Support DetectionMembrane (chemiluminescence) or glass slide (fluorescence)

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EGFR Phosphorylation Array

Assay PrincipleSandwich-based array
Sample TypesLysates
Targets DetectedPhosphorylated EGF receptors
ApplicationDetection of phosphorylation signatures; pathway analysis
Solid Support DetectionMembrane (chemiluminescence)

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MAPK Phosphorylation Array

Assay PrincipleSandwich-based array
Sample TypesLysates
Targets DetectedPhosphorylated human/mouse proteins
ApplicationsDetection of phosphorylation signatures; pathway analysis
Solid Support DetectionMembrane (chemiluminescence)

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Phosphorylation ELISA

Assay PrincipleSandwich-based ELISA
Sample TypesLysates
Targets DetectedPhosphorylated MAPK, Akt, Mek, EGFR, Stat
ApplicationPhospho-protein detection; pathway analysis
Solid Support Detection96-well plate (colorimetric)

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Cell-Based ELISA

Assay PrincipleDirect ELISA
Sample TypesAdherent cell lines
Targets DetectedPhosphorylated MAPK, EGFR, Stat
ApplicationPhospho-protein detection; pathway analysis
Solid Support Detection96-well plate (colorimetric)

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