A Guide to RayBiotech Phosphorylation Assays

Protein phosphorylation plays a prominent role in cell signaling, development and growth. RayBiotech provides a line of phosphorylation immunoassays for monitoring pathway activation, offering a rapid and convenient alternative to standard immunoprecipitations and Western Blots.

AKT Pathway Phosphorylation Array

Assay Principle Sandwich-based array
Sample Types Lysates
Targets Detected Phosphorylated human/mouse proteins involved in AKT pathway
Application Detection of phosphorylation signatures; pathway analysis
Solid Support Detection Membrane (chemiluminescence)

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Apoptosis Signaling Array

Assay Principle Sandwich-based array
Sample Types Lysates
Targets Detected Phosphorylated and cleaved human apoptotic factors
Applications Detection of apoptosis signaling; pathway analysis
Solid Support Detection Membrane (chemiluminescence)

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RTK Phosphorylation Array

Assay Principle Sandwich-based array
Sample Types Lysates
Targets Detected Phosphorylated tyrosine kinase receptors
Applications Detection of phosphorylation signatures; pathway analysis
Solid Support Detection Membrane (chemiluminescence) or glass slide (fluorescence)

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EGFR Phosphorylation Array

Assay Principle Sandwich-based array
Sample Types Lysates
Targets Detected Phosphorylated EGF receptors
Application Detection of phosphorylation signatures; pathway analysis
Solid Support Detection Membrane (chemiluminescence)

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MAPK Phosphorylation Array

Assay Principle Sandwich-based array
Sample Types Lysates
Targets Detected Phosphorylated human/mouse proteins
Applications Detection of phosphorylation signatures; pathway analysis
Solid Support Detection Membrane (chemiluminescence)

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Phosphorylation ELISA

Assay Principle Sandwich-based ELISA
Sample Types Lysates
Targets Detected Phosphorylated MAPK, Akt, Mek, EGFR, Stat
Application Phospho-protein detection; pathway analysis
Solid Support Detection 96-well plate (colorimetric)

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Cell-Based ELISA

Assay Principle Direct ELISA
Sample Types Adherent cell lines
Targets Detected Phosphorylated MAPK, EGFR, Stat
Application Phospho-protein detection; pathway analysis
Solid Support Detection 96-well plate (colorimetric)

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