A Guide to RayBiotech Phosphorylation Assays

Protein phosphorylation plays a prominent role in cell signaling, development and growth. RayBiotech provides a line of phosphorylation immunoassays for monitoring pathway activation, offering a rapid and convenient alternative to standard immunoprecipitations and Western Blots.

Phospho Antibody Arrays Study multiple phosphorylated proteins at one time APPLICATIONS Phospho-protein profiling
Pathway analysis PATHWAYS AKT | Apoptosis | EGFR | Insulin | JAK/STAT | MAPK | NF-κB | RTK | TGFβ SAMPLE TYPES Cell & Tissue lysates
Serum & Plasma
Cell culture supernatants Browse

Sandwich-based Phospho ELISAs Screen different lysates without performing a Western blot analysis APPLICATIONS Total & phospho-protein profiling
Pathway analysis TARGETS Over 150 phospho targets, including ERK1 & 2, JNK, p38, MAPK SAMPLE TYPES Cell & Tissue lysates
Serum & Plasma
Cell culture supernatants Browse

Cell-based Phospho ELISAs Detect phosphorylated proteins in adhered cells APPLICATIONS Total & phospho-protein screening TARGETS Over 75 phospho targets SAMPLE TYPES Adhered cells Browse

Kinase Activity Assays Analyze the activity of kinases APPLICATIONS Screening kinase activity TARGETS AKT | JNK SAMPLE TYPES Cell lysates Browse

References

• Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792. [view publication]
  Product: Human EGFR Phosphorylation Array C1
  Sample Type: Cell Lysate
• Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430 [view publication]
  Product: Human EGFR Phosphorylation Array C1
  Sample Type: Cell Lysate
• Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0. [view publication]
  Product: Human/Mouse MAPK Phosphorylation Array
  Sample Type: Cell Lysate
• Schuett J., Schuett H., Oberoi R., et al. NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells. FASEB J. 2017 Mar 8. pii: fj.201600729R. doi: 10.1096/fj.201600729R [view publication]
  Product: Human/Mouse MAPK Phosphorylation Array
  Sample Type: Cell Lysate
• Al-Maghrebi, May, et al. "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." Naunyn-Schmiedeberg's archives of pharmacology 389.5 (2016): 539-551. [view publication]
  Product: Human/Mouse/Rat Phospho-Jak1 (Y1022) & Total JAK1 ELISA
  Sample Type: Tissue lysate
• Wang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911. [view publication]
  Product: Human RTK Phosphorylation Array C1
  Sample Type: Cell Lysate
• Wang N., Rayes R., Elahi S., et al. The IGF-Trap: Novel Inhibitor of Carcinoma Growth and Metastasis. Mol Cancer Ther. 2015 Apr;14(4):982-93. doi: 10.1158/1535-7163.MCT-14-0751. [view publication]
  Product: Human/Mouse/Rat Phospho-AKT (S473) and Total Akt ELISA
  Sample Type: Cell Lysate
• Al-Gayyar M., et al. Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: Effects on cytokine balance and apoptosis. Life Sciences Available online 23 July 2014. http://dx.doi.org/10.1016/j.lfs.2014.07.002 [view publication]
  Product: Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA
  Sample Type: Tissue Lysate
• Elamin E., et al. Ethanol Impairs Intestinal Barrier Function in Humans through Mitogen Activated Protein Kinase Signaling: A Combined In Vivo and In Vitro Approach. PLOS One, September 16, 2014 DOI: 10.1371/journal.pone.0107421 [view publication]
  Product: Phospho-ERK/JNK/P38α ELISA
  Sample Type: Tissue Lysate
• Katsila T., et al. Circulating pEGFR is a Candidate Response Biomarker of Cetuximab Therapy in Colorectal Cancer. Clinical Cancer Research, October 2014, epub ahead of print. DOI: 10.1158/1078-0432.CCR-14-0361. [view publication]
  Product: Human and Mouse Phospho-EGFR (Y1068) ELISA
  Sample Type: Plasma

More references are listed per product on the individual product pages.