A Guide to RayBiotech Phosphorylation Assays

Protein phosphorylation plays a prominent role in cell signaling, development and growth. RayBiotech provides a line of phosphorylation immunoassays for monitoring pathway activation, offering a rapid and convenient alternative to standard immunoprecipitations and Western blots.

Phospho Antibody Arrays

Study multiple phosphorylated proteins at one time

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Sandwich-based ELISAs

Screen different lysates without performing a Western blot analysis

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Cell-based ELISAs

Detect phosphorylated proteins in adhered cells

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Kinase Activity Assays

Analyze the activity of kinases

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Quantitative Sandwich-based Phospho ELISAs

Determine the concentration of your protein-of-interest

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References

Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792. [view publication]
Product: Human EGFR Phosphorylation Array C1
Sample Type: Cell Lysate
Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430 [view publication]
Product: Human EGFR Phosphorylation Array C1
Sample Type: Cell Lysate
Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0. [view publication]
Product: Human/Mouse MAPK Phosphorylation Array
Sample Type: Cell Lysate
Schuett J., Schuett H., Oberoi R., et al. NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells. FASEB J. 2017 Mar 8. pii: fj.201600729R. doi: 10.1096/fj.201600729R [view publication]
Product: Human/Mouse MAPK Phosphorylation Array
Sample Type: Cell Lysate
Al-Maghrebi, May, et al. "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." Naunyn-Schmiedeberg's archives of pharmacology 389.5 (2016): 539-551. [view publication]
Product: Human/Mouse/Rat Phospho-Jak1 (Y1022) & Total JAK1 ELISA
Sample Type: Tissue lysate
Wang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911. [view publication]
Product: Human RTK Phosphorylation Array C1
Sample Type: Cell Lysate
Wang N., Rayes R., Elahi S., et al. The IGF-Trap: Novel Inhibitor of Carcinoma Growth and Metastasis. Mol Cancer Ther. 2015 Apr;14(4):982-93. doi: 10.1158/1535-7163.MCT-14-0751. [view publication]
Product: Human/Mouse/Rat Phospho-AKT (S473) and Total Akt ELISA
Sample Type: Cell Lysate
Al-Gayyar M., et al. Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: Effects on cytokine balance and apoptosis. Life Sciences Available online 23 July 2014. http://dx.doi.org/10.1016/j.lfs.2014.07.002 [view publication]
Product: Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA
Sample Type: Tissue Lysate
Elamin E., et al. Ethanol Impairs Intestinal Barrier Function in Humans through Mitogen Activated Protein Kinase Signaling: A Combined In Vivo and In Vitro Approach. PLOS One, September 16, 2014 DOI: 10.1371/journal.pone.0107421 [view publication]
Product: Phospho-ERK/JNK/P38Î± ELISA
Sample Type: Tissue Lysate