A Guide to RayBiotech Phosphorylation Assays

Protein phosphorylation plays a prominent role in cell signaling, development and growth. RayBiotech provides a line of phosphorylation immunoassays for monitoring pathway activation, offering a rapid and convenient alternative to standard immunoprecipitations and Western blots.

Phospho Antibody Arrays

Study multiple phosphorylated proteins at one time

APPLICATIONS

Phospho-protein profiling
Pathway analysis

PATHWAYS

SAMPLE TYPES

Cell & Tissue lysates
Serum & Plasma
Cell culture supernatants

Browse

Sandwich-based ELISAs

Screen different lysates without performing a Western blot analysis

APPLICATIONS

Total & phospho-protein profiling
Pathway analysis

TARGETS

Over 150 phospho targets

SAMPLE TYPES

Cell & Tissue lysates
Serum & Plasma
Cell culture supernatants

Browse

Cell-based ELISAs

Detect phosphorylated proteins in adhered cells

APPLICATIONS

Total & phospho-protein screening

TARGETS

Over 75 phospho targets

SAMPLE TYPES

Adhered cells

Browse

Kinase Activity Assays

Analyze the activity of kinases

APPLICATIONS

Screening kinase activity

TARGETS

AKT | JNK

SAMPLE TYPES

Cell lysates

Browse

Quantitative Sandwich-based Phospho ELISAs

Determine the concentration of your protein-of-interest

APPLICATIONS

Total & phospho-protein profiling
Pathway analysis

TARGETS

AKT | Alpha-synuclein | BTK | P706S | SRC | & growing

SAMPLE TYPES

Cell & tissue lysates
Serum & plasma
Cell culture supernatants

Browse

References

Wang X., Zhang M., Ping F., et al. Identification and therapeutic intervention of coactivated anaplastic lymphoma kinase, fibroblast growth factor receptor 2, and ephrin type-A receptor 5 kinases in hepatocellular carcinoma. Hepatology. 2018 Jan 21. doi: 10.1002/hep.29792. [view publication]
Product: Human EGFR Phosphorylation Array C1
Sample Type: Cell Lysate
Ruiz-Saenz A., Dreyer C., Campbell M., et al. HER2 amplification in tumors activates PI3K/Akt signaling independent of HER3. Cancer Research, epub online 2018, doi: 10.1158/0008-5472.CAN-18-0430 [view publication]
Product: Human EGFR Phosphorylation Array C1
Sample Type: Cell Lysate
Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0. [view publication]
Product: Human/Mouse MAPK Phosphorylation Array
Sample Type: Cell Lysate
Schuett J., Schuett H., Oberoi R., et al. NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells. FASEB J. 2017 Mar 8. pii: fj.201600729R. doi: 10.1096/fj.201600729R [view publication]
Product: Human/Mouse MAPK Phosphorylation Array
Sample Type: Cell Lysate
Al-Maghrebi, May, et al. "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." Naunyn-Schmiedeberg's archives of pharmacology 389.5 (2016): 539-551. [view publication]
Product: Human/Mouse/Rat Phospho-Jak1 (Y1022) & Total JAK1 ELISA
Sample Type: Tissue lysate
Wang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911. [view publication]
Product: Human RTK Phosphorylation Array C1
Sample Type: Cell Lysate
Wang N., Rayes R., Elahi S., et al. The IGF-Trap: Novel Inhibitor of Carcinoma Growth and Metastasis. Mol Cancer Ther. 2015 Apr;14(4):982-93. doi: 10.1158/1535-7163.MCT-14-0751. [view publication]
Product: Human/Mouse/Rat Phospho-AKT (S473) and Total Akt ELISA
Sample Type: Cell Lysate
Al-Gayyar M., et al. Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: Effects on cytokine balance and apoptosis. Life Sciences Available online 23 July 2014. http://dx.doi.org/10.1016/j.lfs.2014.07.002 [view publication]
Product: Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA
Sample Type: Tissue Lysate
Elamin E., et al. Ethanol Impairs Intestinal Barrier Function in Humans through Mitogen Activated Protein Kinase Signaling: A Combined In Vivo and In Vitro Approach. PLOS One, September 16, 2014 DOI: 10.1371/journal.pone.0107421 [view publication]
Product: Phospho-ERK/JNK/P38Î± ELISA
Sample Type: Tissue Lysate