Porcine Cytokine Array C1

RayBio® C-Series Porcine Cytokine Array C1 Kit. Detects 48 Porcine Cytokines. Suitable for all liquid sample types.

$438.34  
AAP-CYT-1-2
+

Antigen Information

Accession Number:
A1YM34 | B0FYK2 | B7SLY2 | C0JPM4 | C6JWG4 | D0FHA1 | F1RKV0 | F1S1F8 | F1S278 | F1SM72   View more
Gene Symbols:
ANGPT1 | CCL4 | CCL5 | CSF2 | CXCL8 | CXCL9 | DCN | EPO | FGF21 | IFN-BETA   View more

Assay Format

Number of Targets Detected:
48
Species Detected:
  • Porcine (pig)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4 or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information

Angiopoietin-1
CCL3L1
Decorin
Eotaxin-1
(CCL11)
Erythropoietin
  • Gene Symbol: EPO
  • Gene ID: 397249
  • Accession #: P49157
  • ELISA Kit: RayBio
FGF-21
  • Gene Symbol: FGF21
  • Gene ID: 100302504
  • Accession #: C6JWG4
  • ELISA Kit: RayBio
Galectin-9
GASP-1
(WFIKKNRP)
GM-CSF
IFN alpha
IFN-beta
  • Gene Symbol: interferon-beta-1 IFN-beta IFNB IFNB1 interferon-beta/ poIFN-beta
  • Accession #: Q29412
  • ELISA Kit: RayBio
IFN-gamma
IGF-2
  • Gene Symbol: IGF2
  • Gene ID: 396916
  • Accession #: P23695
  • ELISA Kit: RayBio
IGFBP-5
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 Ra
(IL-1 F3)
IL-10
  • Gene Symbol: IL10
  • Gene ID: 397106
  • Accession #: Q29055
  • ELISA Kit: RayBio
IL-12 p70
IL-13
IL-15
IL-17A
IL-17F
IL-18
IL-21
  • Gene Symbol: IL21
  • Gene ID: 403123
  • Accession #: Q76LU6
  • ELISA Kit: RayBio
IL-22
IL-28B
  • Gene Symbol: IL28B
  • Gene ID: 100310828
  • Accession #: D0FHA1
  • ELISA Kit: RayBio
IL-4
IL-6
IL-8
(CXCL8)
Insulin
MIF
MIG
(CXCL9)
MIP-1 beta
(CCL4)
NCAM-1
(CD56)
Osteoprotegerin
(TNFRSF11B)
PDGF-BB
PECAM-1
(CD31)
PLGF-2
RANTES
(CCL5)
SCF
  • Gene Symbol: KITLG MGF
  • Gene ID: 100626805 397509
  • Accession #: Q29030
  • ELISA Kit: RayBio
TGF alpha
TGF beta 1
  • Gene Symbol: TGFB1
  • Gene ID: 397078
  • Accession #: P07200
  • ELISA Kit: RayBio
TIMP-1
TIMP-2
TNF alpha
TWEAK R
(TNFRSF12)
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Porcine Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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