Porcine Cytokine Array Q1

Quantibody® Porcine (pig) Cytokine Array 1 Kit. Detects 10 Porcine (pig) Cytokines. Suitable for all liquid sample types.

$428.24
QAP-CYT-1-1
+

Antigen Information

Gene Symbols:
  • CSF2
  • CXCL8
  • IFNG
  • IL10
  • IL12A
  • IL1B
  • IL4
  • IL6
  • TGFB1
  • TNF

Assay Format

Pathway:
  • JAK/STAT Signaling
  • MAPK Signaling
  • NFkB Signaling
Number of Targets Detected:
10
Species Detected:
  • Porcine (pig)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
IFN-gamma
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
IL-4
IL-6
IL-8
(CXCL8)
TGF beta 1
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Porcine Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Sohn EH., Jiao C., Kaalberg E., et al. Allogenic iPSC-derived RPE cell transplants induce immune response in pigs: a pilot study. Sci Rep. 2015 Jul 3;5:11791. doi: 10.1038/srep11791.
    Species: Porcine
    Sample type: Vitreous Fluid
  2. Goetz J., Fredericks D., Petersen E., et al. A clinically realistic large animal model of intra-articular fracture that progresses to post-traumatic osteoarthritis. Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805. doi: 10.1016/j.joca.2015.05.022
    Species: Porcine
    Sample type: Synovial Fluid
  3. Beltran-Beck B., de la Fuente J., Garrido J., Aranaz A., Sevilla I., et al. Oral Vaccination with Heat Inactivated Mycobacterium bovis Activates the Complement System to Protect against Tuberculosis. PLOS One, 2014 May 19, DOI: 10.1371/journal.pone.0098048
    Species: Porcine
    Sample type: Serum
  4. Beltran-Beck B., Romero B., Boadella M., et al. Tonsils of the Soft Palate Do Not Mediate the Response of Pigs to Oral Vaccination with Heat-Inactivated Mycobacterium bovis. Clin Vaccine Immunol. 2014 Aug;21(8):1128-36. doi: 10.1128/CVI.00221-14
    Species: Porcine
    Sample type: Serum
  5. Butz D., et al. The expired breath carbon delta value is a marker for the onset of sepsis in a swine model. Journal of Analytical Atomic Spectrometry. 2014, 29, 606-613. DOI: 10.1039/C3JA50340B
    Species: Porcine
    Sample type: Plasma
  6. Pazos, M. R., Mohammed, N., Lafuente, H., Santos, M., Martínez-Pinilla, E., Moreno, E., ... & Hillard, C. J. (2013). Mechanisms of cannabidiol neuroprotection in hypoxic–ischemic newborn pigs: Role of 5HT 1A and CB2 receptors. Neuropharmacology, 71, 282-291.
    Species: Porcine
    Sample type: Tissue Lysate
  7. Mulloy DP, Stone ML, Crosby IK, et al. Ex Vivo Rehabilitation of Non-Heart-Beating Donor Lungs in a Preclinical Porcine Model: Delayed Perfusion Results in Superior Lung Function. The Journal of thoracic and cardiovascular surgery. 2012;144(5):1208-1216. doi:10.1016/j.jtcvs.2012.07.056.
    Species: Porcine
    Sample type: BAL Fluid
Sensitive and robust with good reproducibility. We took advantage of the free scanning services as well. Low price in comparison to others in the market.

University of Memphis
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Q-Analyzer Bundle Discount

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1 x $428.24
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70% off the Q-Analyzer tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department ([email protected]).
Total list price $638.32
Price for all $491.26

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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