Transcription Factor ELISA Detection
 

Transcription Factor Activity Assay

The RayBio® Transcription Factor-Activity Assays are non-radioactive transcription factor activity assays with an ELISA format. They offer an easy, speedy, sensitive and high-throughput method to detect the activation of transcription factors. Double stranded oligonucleotides are coated in a 96-well plate. These oligonucleotides specifically capture the target transcription factor contained in whole cell lysate or nuclear extracts after a short incubation. Subsequently, the primary antibody recognizes the target transcription factor-DNA complex in each well, and a HRP-conjugated secondary antibody is then used for detection. After washing away any unbound antibody, signal can be obtained easily through a colorimetric assay with a spectrophotometric plate reader at 450 nm. The specificity of the reaction between the target transcription factor and the DNA probe is additionally stringent because of the establishment of specific competitive DNA and non-specific competitive DNA probes in this reaction system.

RayBio® Human c-Fos Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Fos binding sequences to semi-quantitatively detect active c-Fos in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: FOS, G0S7 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-CFOS-1
$402.00 $321.60

RayBio® Human c-Myc Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Myc binding sequences to semi-quantitatively detect active c-Myc in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: BHLHE39, MYC Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-CMYC-1
$402.00 $321.60

RayBio® Human ELK-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ELK-1 binding sequences to semi-quantitatively detect active ELK-1 in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: ELK1 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-ELK1-1
$402.00 $321.60

RayBio® Human ER-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical ER-alpha binding sequences to semi-quantitatively detect active ER-alpha in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: ESR, ESR1, NR3A1 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-ERa-1
$402.00 $321.60

RayBio® Human Fra1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical Fra1 binding sequences to semi-quantitatively detect active Fra1 in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: FOSL1, FRA1 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-FRA1-1
$402.00 $321.60

RayBio® Human fosB Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical fosB binding sequences to semi-quantitatively detect active fosB in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: FOSB, G0S3 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-FOSB-1
$402.00 $321.60

RayBio® Human c-Jun Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical c-Jun binding sequences to semi-quantitatively detect active c-Jun in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: JUN Target Name: c-Jun Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-CJUN-1
$402.00 $321.60

RayBio® Human FRA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical FRA-2 binding sequences to semi-quantitatively detect active FRA-2 in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: FOSL2 
TFEH-FRA2-1
$402.00 $321.60

RayBio® Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: ERYF1, GATA1, GF1 Size: 1, 2, or 5 x 96-Well Strip Microplate Kit 
TFEH-GATA1-1
$402.00 $321.60

RayBio® Human GATA-2 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA-2 binding sequences to semi-quantitatively detect active GATA-2 in lysates or nuclear extracts. Dry ice shipment (additional fee).

Gene Symbols: GATA2 
TFEH-GATA2-1
$402.00 $321.60