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Assay Format

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:

AXL | BTC | CCL13 | CCL14 | CCL16 | CCL17 | CCL18 | CCL19 | CCL20 | CCL21 View more

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
6Ckine (CCL21) Gene Symbol: CCL21 Gene ID: 6366 Accession #: O00585 ELISA Kit: RayBio Human 6Ckine ELISA	Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA
CXCL16 Gene Symbol: CXCL16 Gene ID: 58191 Accession #: Q9H2A7 ELISA Kit: RayBio Human CXCL16 ELISA	ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	GCP-2 (CXCL6) Gene Symbol: CXCL6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GRO Gene Symbol: CXCL1/CXCL2/CXCL3
HCC-1 (CCL14) Gene Symbol: CCL14 Gene ID: 6358 Accession #: Q16627 ELISA Kit: RayBio Human HCC-1 ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA	IL-9 Gene Symbol: IL9 Gene ID: 3578 Accession #: P15248 ELISA Kit: RayBio Human IL-9 ELISA	IL-17F Gene Symbol: IL17F Gene ID: 112744 Accession #: Q96PD4 ELISA Kit: RayBio Human IL-17F ELISA	IL-18 BP alpha Gene Symbol: IL18BP Gene ID: 10068 Accession #: O95998 ELISA Kit: RayBio Human IL-18 BPa ELISA
IL-28A Gene Symbol: IFNL2 Gene ID: 282616 Accession #: Q8IZJ0 ELISA Kit: RayBio Human IL-28A ELISA	IL-29 Gene Symbol: IFNL1 Gene ID: 282618 Accession #: Q8IU54 ELISA Kit: RayBio Human IL-29 ELISA	IL-31 Gene Symbol: IL31 Gene ID: 386653 Accession #: Q6EBC2 ELISA Kit: RayBio Human IL-31 ELISA	IP-10 (CXCL10) Gene Symbol: CXCL10 Gene ID: 3627 Accession #: P02778 ELISA Kit: RayBio Human IP-10 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA
LIF Gene Symbol: LIF Gene ID: 3976 Accession #: P15018 ELISA Kit: RayBio Human LIF ELISA	Light (TNFSF14) Gene Symbol: TNFSF14 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA
MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA	MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA
MPIF-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human MPIF-1 ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: Ppbp Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA
Platelet Factor 4 (CXCL4) Gene Symbol: PF4 CXCL4 SCYB4 Gene ID: 5196 Accession #: P02776 ELISA Kit: RayBio Human PF-4 ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA	TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TSLP Gene Symbol: TSLP Gene ID: 85480 Accession #: Q969D9 ELISA Kit: RayBio Human TSLP ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Chemokine Array Q1 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.