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Human Cytokine Array Q4000

Quantibody® Human Cytokine Array 4000 Kit. A combination of Human Inflammation Array Q3, Human Growth Factor Array Q1, Human Chemokine Array Q1, Human Receptor Array Q1, and Human Cytokine Array Q4. Detects 200 Human Inflammatory Factors, Growth Factors, Chemokines, Receptors, and Cytokines. Suitable for all liquid sample types.

Old price: $4,950.40
$3,960.32  
You save: $990.08 (20%)
QAH-CAA-4000-1
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Antigen Information

Gene Symbols:
AGRP | ALCAM | ANG | ANGPT1 | AREG | AXL | BDNF | BMP4 | BMP5 | BMP7   View more

Assay Format

Number of Targets Detected:
200
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 250 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), Activin A, AgRP, ALCAM (CD166), Amphiregulin, Angiogenin, Angiopoietin-1, Angiostatin, AXL, BCMA, BDNF, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-4, BMP-5, BMP-7, Cathepsin S, CCL28 (MEC), CD14, CD30 (TNFRSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5), CD80 (B7-1), CEACAM-1, Contactin-2 (TAG1), Cripto-1, CTACK (CCL27), CXCL16, DAN, DKK-1, DR6 (TNFRSF21), Dtk, E-Cadherin, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB3, E-Selectin, Fas (TNFRSF6/Apo-1), Fas Ligand (TNFSF6), Fc gamma RIIB/C (CD32B/C), FGF-4, FGF-7 (KGF), Flt-3 Ligand, Follistatin, Galectin-7, GCP-2 (CXCL6), GCSF, GDF-15, GDNF, GITR (TNFRSF18), GM-CSF, gp130 (sgp130), GRO alpha/beta/gamma, Growth Hormone, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), HGF, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R4 (ST2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-16, IL-17 RA, IL-17A, IL-17B, IL-17F, IL-18 BP alpha, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-21 R, IL-23, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-31, IL-4, IL-5, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), LAP/TGF beta 1 (TGF beta 1), LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MICA, MICB, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NGFR (TNFRSF16), NrCAM, NRG1-beta 1 (HRG1-beta 1), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, Platelet Factor 4 (CXCL4), PLGF, RAGE, RANTES (CCL5), Resistin, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), Siglec-5 (CD170), Sonic Hedgehog N-Terminal (Shh-N), TARC (CCL17), TECK (CCL25), TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin (TPO), Tie-2, TIM-1 (KIM-1), TIMP-1, TIMP-2, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), Trappin-2, TREM-1, TROP1 (EpCAM), TSLP, uPAR, VCAM-1 (CD106), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, XEDAR

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
  • Biomarker Validation
  • Validation of Antibody Array Results
  • Quantitative Protein Detection
  • Establishing Normal Range
Kit Components
  • Human Cytokine Array Q4000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, ore the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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  1. Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1.
    Species: Human
    Sample type: Serum (Serum for melanoma patients undergoing immunotherapy)
  2. Martinaud C., Desterke C., Konopacki J., et al. Osteogenic Potential of Mesenchymal Stromal Cells Contributes to Primary Myelofibrosis. Cancer Res. 2015 Nov 15;75(22):4753-65. doi: 10.1158/0008-5472.CAN-14-3696
    Species: Human
    Sample type: Conditioned Media (Mesencymal Stromal Cells)
  3. Velez G., Bassuk A., Colgan D., et al. Therapeutic drug repositioning using personalized proteomics of liquid biopsies. JCI Insight. 2017 Dec 21;2(24). pii: 97818. doi: 10.1172/jci.insight.97818.
    Species: Human
    Sample type: Vitreous Fluid (Vitreous sample from retinopathy patients)
  4. Velez G., Roybal C., Colgan D., et al. Precision Medicine: Personalized Proteomics for the Diagnosis and Treatment of Idiopathic Inflammatory Disease. JAMA Ophthalmol. 2016 Apr;134(4):444-8. doi: 10.1001/jamaophthalmol.2015.5934.
    Species: Human
    Sample type: Vitreous Fluid (Human Vitreous Fluid samples)
  5. Yao Y, Yang C, Yi X, Xie S, Sun H. Comparative analysis of inflammatory signature profiles in eosinophilic and noneosinophilic chronic rhinosinusitis with nasal polyposis. Biosci Rep. 2020 Feb 28;40(2):BSR20193101. doi: 10.1042/BSR20193101. PMID: 32039442; PMCID: PMC7040463.
    Species: Human
    Sample type: Tissue Lysate
  6. Ragni E, Perucca Orfei C, De Luca P, Colombini A, Viganò M, de Girolamo L. Secreted Factors and EV-miRNAs Orchestrate the Healing Capacity of Adipose Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis. Int J Mol Sci. 2020 Feb 26;21(5):1582. doi: 10.3390/ijms21051582. PMID: 32111031; PMCID: PMC7084308.
    Species: Human
    Sample type: Conditioned Media (ASC secretomes)
It is easy to succeed to complete the protocol. The details of the instruction help a lot.

University of Nebraska-Lincoln
We had RayBiotech run this panel on a collection of human brain samples. The results are terrific and working with Raybiotech has been great.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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