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Human IGF Signaling Array Q1

Quantibody® Human Insulin-like Growth Factor Signaling Array 1 Kit. Detects 10 Human Cytokines. Suitable for all liquid sample types.

Old price: $285.83
$200.08  
You save: $85.75 (30%)
QAH-IGF-1-1
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Antigen Information

Gene Symbols:
  • IGF1
  • IGF1R
  • IGF2
  • IGF2R
  • IGFBP1
  • IGFBP2
  • IGFBP3
  • IGFBP4
  • IGFBP6
  • INS

Assay Format

Pathway:
  • Akt Signaling
  • IGF Signaling
  • p53 Signaling
  • PI3K-AKT Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IGF-1
IGF-1 R
IGF-2
  • Gene Symbol: Igf2
  • Gene ID: 3481
  • Accession #: P01344
IGF-2 R
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
Insulin

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human IGF-1 Signaling Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
This is an awesome product from raybiotech. We studied this panel of different proteins in one go and it was pretty easy to analyse and compare the results with treatments. I would definitely recommend this for studying the pathway as it features a good panel of receptors & secreted proteins.

Moffitt Cancer center
Great product for targeted proteomics! Simple to use and could quickly run 16 samples per slide. Could see clear differences between sample supernatants as desired. The tech support team was also wonderful in helping with the data analysis. Would definitely recommend for anyone studying this pathway as it features both receptors and secreted proteins.

Columbia University
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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