Human MMP Array Q1

Quantibody® Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs. Suitable for all liquid sample types.

$420.00  
QAH-MMP-1-1
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Assay Format

Pathway:
  • Akt Signaling
  • HIF-1 alpha Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:
  • MMP1
  • MMP10
  • MMP13
  • MMP2
  • MMP3
  • MMP8
  • MMP9
  • TIMP1
  • TIMP2
  • TIMP4

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
MMP-1
MMP-10
MMP-13
MMP-2
MMP-3
MMP-8
MMP-9
TIMP-1
TIMP-2
TIMP-4

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human MMP Array Q1 Slide(s)
  • Sample Diluent
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Cekanova M., Fernando R., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016.
    Species: Human
    Sample type: Conditioned Media (Human MCF-7 cells silenced with BAD siRNA)
  2. Yang C-C, Lin C-Y, Wang H-S, Lyu S-R (2013) Matrix Metalloproteases and Tissue Inhibitors of Metalloproteinases in Medial Plica and PannuslikeTissue Contribute to Knee Osteoarthritis Progression. PLoS ONE 8(11): e79662. doi:10.1371/journal.pone.0079662
    Species: Human
    Sample type: Tissue Lysate (Medial plica and pannus tissue from OA patients)
  3. Yang, Chih-Chang, et al. "Matrix metalloproteases and tissue inhibitors of metalloproteinases in medial plica and pannus-like tissue contribute to knee osteoarthritis progression." PLoS One 8.11 (2013): e79662.
    Species: Human
    Sample type: Tissue Lysate (OA medial plica and pannus-like tissue)
  4. Wang Y., et al. Circulating matrix metalloproteinase patterns in association with aortic dilatation in bicuspid aortic valve patients with isolated severe aortic stenosis. Heart and Vessels, October 2014, epub ahead of print. DOI 10.1007/s00380-014-0593-5
    Species: Human
    Sample type: Plasma
  5. Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6
    Species: Human
    Sample type: Conditioned Media (MDA-MB-231 cells)
  6. Fa, Ming, et al. "Novel approach for quantitative measurement of matrix metalloprotease-1 (MMP1) in human breast cancer cells using mass spectrometry." Journal of Analytical Sciences, Methods and Instrumentation 3.1 (2013): 54.
    Species: Human
    Sample type: Cell Lysate (SKBR3 cancer cells)
This array, like their other Q-type products, worked well, was easy to use, and provided a lot of data for my samples at a great cost relative to competitors. I successfully ran supernatants from functional engineered bone tissues and cancer cell lines. The data analysis by the manufacturer was quick as always. As someone with expertise in cancer cell and engineered tissue culture, I would highly recommend this product.

Columbia University
The end user in our lab had the following to say: "I would rate it 5/5. Works as described. Affordable."

Hershey Medical Center
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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