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Assay Format

Pathway:

Akt Signaling
HIF-1 alpha Signaling

Number of Targets Detected:

Species Detected:

Human

Compatible Sample Types:

Cell Culture Supernatants
Cell Lysates
Other Body Fluids
Plasma
Serum
Tissue Lysates

Design Principle:

Sandwich-based

Method of Detection:

Fluorescence Laser Scanner

Quantitative/Semi-Quantitative:

Quantitative

Solid Support:

Glass Slide

Product Specifications

Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:

MMP1
MMP10
MMP13
MMP2
MMP3
MMP8
MMP9
TIMP1
TIMP2
TIMP4

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
MMP-1 Gene Symbol: MMP1 Gene ID: 4312 Accession #: P03956 ELISA Kit: RayBio Human MMP-1 ELISA	MMP-10 Gene Symbol: MMP10 Gene ID: 4319 Accession #: P09238 ELISA Kit: RayBio Human MMP-10 ELISA	MMP-13 Gene Symbol: MMP13 Gene ID: 4322 Accession #: P45452 ELISA Kit: RayBio Human MMP-13 ELISA	MMP-2 Gene Symbol: MMP2 Gene ID: 4313 Accession #: P08253 ELISA Kit: RayBio Human MMP-2 ELISA	MMP-3 Gene Symbol: MMP3 Gene ID: 4314 Accession #: P08254 ELISA Kit: RayBio Human MMP-3 ELISA
MMP-8 Gene Symbol: MMP8 Gene ID: 4317 Accession #: P22894 ELISA Kit: RayBio Human MMP-8 ELISA	MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TIMP-4 Gene Symbol: TIMP4 Gene ID: 7079 Accession #: Q99727 ELISA Kit: RayBio Human TIMP-4 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human MMP Array Q1 Slide(s)
Sample Diluent
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Sood D, Tang-Schomer M, Pouli D, Mizzoni C, Raia N, Tai A, Arkun K, Wu J, Black LD 3rd, Scheffler B, Georgakoudi I, Steindler DA, Kaplan DL. 3D extracellular matrix microenvironment in bioengineered tissue models of primary pediatric and adult brain tumors. Nat Commun. 2019 Oct 4;10(1):4529. doi: 10.1038/s41467-019-12420-1. PMID: 31586101; PMCID: PMC6778192.
Species: Human
Sample type: Conditioned Media
Sava P., Ramanathan A., Dobronyi A., et al. Human pericytes adopt myofibroblast properties in the microenvironment of the IPF lung. JCI Insight. 2017 Dec 21;2(24). pii: 96352. doi: 10.1172/jci.insight.96352
Species: Human
Sample type: Conditioned Media
Cekanova M., Feranando RI., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016.
Species: Human
Sample type: Cell Lysate
Collazos J., Asensi V., Martin G., et al. The effect of gender and genetic polymorphisms on matrix metalloprotease (MMP) and tissue inhibitor (TIMP) plasma levels in different infectious and non-infectious conditions. Clin Exp Immunol. 2015 Nov;182(2):213-9. doi: 10.1111/cei.12686.
Species: Human
Sample type: Plasma
Tian F., Qin C., Li X., et al. Decreased stathmin-1 expression inhibits trophoblast proliferation and invasion and is associated with recurrent miscarriage. Am J Pathol. 2015 Oct;185(10):2709-21. doi: 10.1016/j.ajpath.2015.06.010.
Species: Human
Sample type: Conditioned Media
Cekanova M., Fernando R., Siriwardhana N., et al. BCL-2 family protein, BAD is down-regulated in breast cancer and inhibits cell invasion. Exp Cell Res. 2015 Feb 1;331(1):1-10. doi: 10.1016/j.yexcr.2014.11.016.
Species: Human
Sample type: Conditioned Media
Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6
Species: Human
Sample type: Conditioned Media
Epanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanism
Species: Human
Sample type: Conditioned Media
Martin G., Asensi V., Montes H., Collazos J., Alvarez V., Carton J., Taboada F., Valle-Garay E. Role of plasma matrix-metalloproteases (MMPs) and their polymorphisms (SNPs) in sepsis development and outcome in ICU patients. Scientific Reports 4, 5002. doi:10.1038/srep05002.
Species: Human
Sample type: Plasma
Wang Y., et al. Circulating matrix metalloproteinase patterns in association with aortic dilatation in bicuspid aortic valve patients with isolated severe aortic stenosis. Heart and Vessels, October 2014, epub ahead of print. DOI 10.1007/s00380-014-0593-5
Species: Human
Sample type: Plasma

1 2

This array, like their other Q-type products, worked well, was easy to use, and provided a lot of data for my samples at a great cost relative to competitors. I successfully ran supernatants from functional engineered bone tissues and cancer cell lines. The data analysis by the manufacturer was quick as always. As someone with expertise in cancer cell and engineered tissue culture, I would highly recommend this product.

AC @ Columbia
Columbia University 04/12/2021, 02:45 PM

The end user in our lab had the following to say: "I would rate it 5/5. Works as described. Affordable."

Kathryn Gilliland
Hershey Medical Center 08/08/2018, 05:42 AM

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