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Human Th1/Th2 Array Q1

Quantibody® Human Th1/Th2 Array 1 Kit. Detects 10 Human Th1 and Th2 Cytokines. Suitable for all liquid sample types.

Old price: $202.00
$141.40  
You save: $60.60 (30%)
QAH-TH-1-1
+

Antigen Information

Gene Symbols:
  • CSF2
  • CXCL8
  • IFNG
  • IL10
  • IL13
  • IL2
  • IL4
  • IL5
  • IL6
  • TNF

Assay Format

Pathway:
  • JAK/STAT Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
IFN-gamma
IL-10
IL-13
IL-2
IL-4
IL-5
IL-6
IL-8
(CXCL8)
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Th1/Th2 Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Schwarz C, Klaus DA, Tudor B, Fleischmann E, Wekerle T, Roth G, et al. (2015)Transection Speed and Impact on Perioperative Inflammatory Response - A Randomized Controlled Trial Comparing Stapler Hepatectomy and CUSA Resection. PLoS ONE 10(10): e0140314. doi:10.1371/journal.pone.0140314
    Species: Human
    Sample type: Serum
  2. Chia C., Kumari U., Casey P. Breast cancer cell invasion mediated by Galpha12 signaling involves expression of interleukins-6 and -8, and matrix metalloproteinase-2. Journal of Molecular Signaling 2014, 9:6 doi:10.1186/1750-2187-9-6
    Species: Human
    Sample type: Conditioned Media
  3. Epanchintsev A., Pavithra S., Verma R., Lyakhovich A. IL-6, IL-8, MMP-2, MMP-9 are overexpressed in Fanconi anemia cells through a NF-?B/TNF-? dependent mechanism
    Species: Human
    Sample type: Conditioned Media
  4. Veiga P., Schroth R., Guedes R., Freire S., Nogueira-Filho G., Serum cytokine profile among Brazilian children of African descent with periodontal inflammation and sickle cell anaemia. Archives of Oral Biology Volume 58, Issue 5, May 2013, Pages 505-510
    Species: Human
    Sample type: Serum
  5. Knotkova, H. et al. Using Transcranial Direct Current Stimulation to Treat Depression in HIV-Infected Persons: The Outcomes of a Feasibility Study. Front Psychiatry. 2012 Jun 18;3:59. doi: 10.3389/fpsyt.2012.00059. eCollection 2012.
    Species: Human
    Sample type: Serum
  6. Green V., Michno A., Greenman J., Stafford N. Effect of treatment on systemic cytokines in head and neck squamous cell carcinoma patients. Results Immunol. 2012; 2: 1-6. Published online 2011 Dec 17. doi: 10.1016/j.rinim.2011.12.001
    Species: Human
    Sample type: Serum
  7. Stechova K., Halbhuber Z., Hubackova M., et al. Case report: type 1 diabetes in monozygotic quadruplets. Eur J Hum Genet. 2012 Apr;20(4):457-62. doi: 10.1038/ejhg.2011.212.
    Species: Human
    Sample type: Conditioned Media
  8. Fleming, J. O., et al. "Probiotic helminth administration in relapsing?remitting multiple sclerosis: a phase 1 study."?Multiple Sclerosis Journal?17.6 (2011): 743-754.
    Species: Human
    Sample type: Serum
  9. Gao QT, Cheung JKH, Li J, Jiang ZY, Chu GKY, et al. A Chinese herbal decoction, Danggui Buxue Tang, activates extracellular signal-related kinase in cultured T-lymphocytes. FEBS Lett. 2007;581: 5087-5099.
    Species: Human
    Sample type: Conditioned Media
Good experience overall! I had some problems visualizing the expression of the cytokines on my samples, but the staff from RayBiotech extracted the data for me at no extra cost and fixed the problem.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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