Mouse Th1/Th2/Th17 Array Q1

Quantibody® Mouse Th17 Array 1 Kit. Detects 18 Mouse Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.

$420.00  
QAM-TH17-1-1
+

Assay Format

Number of Targets Detected:
18
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information

Gene Symbols:
CCL20 | IFNG | IFNL2 | IL10 | IL12A | IL13 | IL17A | IL17F | IL1B | IL2   View more

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
IFN-gamma
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-17A
IL-17F
IL-2
IL-21
IL-22
IL-23
IL-28A
(IFN-lambda 2)
IL-4
IL-5
IL-6
MIP-3 alpha
(CCL20)
TGF beta 1
  • Gene Symbol: TGFB1
  • Accession #: P04202
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Mouse Th1/Th2/Th17 Array Q2 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Velsko I., Chukkapalli S., Rivera-Kweh M., et al. Periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in ?v?6 integrin-deficient mice. Infect Immun. 2015 Dec;83(12):4582-93. doi: 10.1128/IAI.01077-15.
    Species: Human
    Sample type: Conditioned Media
  2. Laky N., Evans S., Perez-Diez A., Fowlkes B. Notch signaling regulates antigen sensitivity of naive CD4+ T cells by tuning co-stimulation. Immunity . 2015 Jan 20;42(1):80-94. doi: 10.1016/j.immuni.2014.12.027. Epub 2015 Jan 1.
    Species: Mouse
    Sample type: Conditioned Media
  3. Che N. et al. Impaired B cell Inhibition by Lupus Bone Marrow Mesenchymal Stem Cells is Caused by Reduced CCL2 Expression. J Immunol 2014; 193:5306-5314; Prepublished online 22. doi: 10.4049/jimmunol.1400036
    Species: Mouse
    Sample type: Conditioned media
  4. Hou T., Li Z., Luo F., Xie Z., Wu X., Xing J., Dong S., Xu J. A composite demineralized bone matrix e Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow. Biomaterials, Available online 19 April 2014. [Epub ahead of print]
    Species: Mouse
    Sample type: Tissue Lysate
  5. Hou, Ni, et al. "A novel chronic stress-induced shift in the Th1 to Th2 response promotes colon cancer growth." Biochemical and biophysical research communications 439.4 (2013): 471-476.
    Species: Mouse
    Sample type: Serum
  6. Marchesan, Julie Teresa, et al. "Porphyromonas gingivalis oral infection exacerbates the development and severity of collagen-induced arthritis." Arthritis research & therapy 15.6 (2013): R186.
    Species: Mouse
    Sample type: Serum
  7. Abdi K., Sing N., Matzinger P. Lipopolysaccharide-activated dendritic cells: "exhausted" or alert and waiting? J Immunol. 2012 Jun 15;188(12):5981-9. doi: 10.4049/jimmunol.1102868.
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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