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Quantibody: Multiplex ELISA Array

Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Quantibody® is an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types. Quantibody® combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array.

With this platform, only 50 µl of sample is needed for quantification of up to 40 proteins in quadruplicate (0.5 ml for the 400 target Quantibody array), making this array 80 times more efficient than traditional ELISA.

For a comparison & overview of all our antibody array systems, click here.

Compare Quantibody Protein ListsCustomize Your ArrayBrowse Quantibody Arrays

Quantiative Proteomics Services

High-Density Quantibody Full Testing Services

Ship us your samples (5 sample minimum) and our experienced service department will use our GLP-compliant Quantibody® array platform to quantitatively detect up to 1000 human, 200 mouse, or 67 rat proteins and return to you a full report of your results. Click the button below to learn more.

Quantitative Proteomics Services

Quantibody Features

Get 4x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with 16 identical antibody arrays (also called  “subarrays”). Within each subarray, the capture antibodies along with controls are spotted in quadruplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 14 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

Multichannel Pipette

Features

  • Less sample, more data: only 50 ul of sample is needed for quantification of up to 40 cytokines
  • 4-hour processing time
  • More cost-effective than ELISA
  • No interference between capture antibodies (unlike bead-based multiplex assays)
  • No dedicated equipment required
  • Customizable – create a custom array from our list of targets

Contents of kit

  • Glass Chip with antibody arrays
  • Sample Diluent
  • Lyophilized protein standard mix
  • Detection antibody cocktail
  • Streptavidin-Fluorescent dye
  • Wash buffer
  • Manual

*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

How It Works

Quantibody Multiplex ELISA Array - How it works

Like a traditional sandwich-based ELISA, Quantibody uses a matched pair of antibodies for target protein detection.  A panel of capture antibodies is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

Quantibody Standard Curves

To quantify target protein concentrations, the array-specific protein standards, whose concentrations have been predetermined, are provided to generate an 8-point standard curve of each target protein. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Quantibody slide scanning and data extraction service is now FREE for a limited time only!

Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Q-Analyzer Tool specific for your array. The Q-Analyzer will automatically plot the standard curve for each analyte as well as perform background subtraction and normalization.

Research Applications

  • High-throughput profiling of cytokine expression
  • Validation of semi-quantitative antibody array results
  • Identifying potential molecular targets for drug development
  • Identifying the molecular mechanisms of drug action
  • Identifying crucial factors involved in disease processes
  • Discovering biomarkers for disease management
  • Discovering expression patterns for molecular classification of diseases

Multiplex Protein Detection with Quantibody® Arrays

References

  1. Zeng Q., et al. The functional behavior of a macrophage/fibroblast co-culture model derived from normal and diabetic mice with a marine gelatin-oxidized alginate hydrogel. Biomaterials. 2010 Aug;31(22):5772-81. doi: 10.1016/j.biomaterials.2010.04.022.
    Species:Mouse
  2. Toh H, Wang W, Chia W, Kvistborg P, Sun Li,et al. Clinical Benefit of Allogeneic Melanoma Cell Lysate-Pulsed Autologous Dendritic Cell Vaccine in MAGE-Positive Colorectal Cancer Patients.Clin Cancer Res. 2009;15(24):7726-7736
    Species:Human
    Sample Type:Plasma
  3. Du Y, Wei X, He Y, Wei G, Hampel H, et al. P2-380: Identification and characterization of human autoantibodies that may be used for the treatment of prion diseases. Alzheimer Dementia. 2008;4(4 Suppl):T484 (Abstract P2-380).
    Species:Human
    Sample Type:Plasma
  4. Jonnalagadda D., et al. Platelet secretion is kinetically heterogeneous in an agonist-responsive manner. December 20, 2012; Blood: 120 (26). http://dx.doi.org/10.1182/blood-2012-07-445080
    Species:Human
    Sample Type:Conditioned Media
  5. Vargas-Inchaustegui D., Hogg A., Tulliano G., et al.CXCL10 Production by Human Monocytes in Response to Leishmania braziliensis Infection. Infect. Immun. January 2010 vol. 78 no. 1 301-308
    Species:Human
    Sample Type:Serum
  6. Zhai Y, Zhong Z, Chen C-YA, Xia Z, Song L, Blackburn MR, Shyu A-B. Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation. Mol Cell Biol. 2008; 28(24):7414-7426.
    Species:Human
  7. Huggenberger R., et al. Stimulation of lymphangiogenesis via VEGFR-3 inhibits chronic skin inflammation. J Exp Med. 2010 Sep 27;207(10):2255-69. doi: 10.1084/jem.20100559.
    Species:Mouse
    Sample Type:Tissue Lysate
  8. Jurk D., Wilson C., Passos J., et al. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice. Nature Communications 2, Article number: 4172. doi:10.1038/ncomms5172
    Species:Mouse
    Sample Type:Conditioned Media
  9. Bethunaickan, R., Sahu, R., Liu, Z., Tang, Y. T., Huang, W., Edegbe, O., Tao, H., Ramanujam, M., Madaio, M. P. and Davidson, A. (2012), Anti-tumor necrosis factor alpha treatment of IFN-alpha-induced murine lupus nephritis reduces the renal macrophage response but does not alter glomerular immune complex formation. Arthritis & Rheumatism, 64: 3399-3408. doi: 10.1002/art.34553
    Species:Mouse
    Sample Type:Tissue Lysate
  10. Hou T., Li Z., Luo F., Xie Z., Wu X., Xing J., Dong S., Xu J. A composite demineralized bone matrix e Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow. Biomaterials, Available online 19 April 2014. [Epub ahead of print]
    Species:Mouse
    Sample Type:Tissue Lysate
  11. Feng W., Madajka M., Kerr B., Mahabeleshwar G., White S., Byzova T. A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing. Blood April 7, 2011 vol. 117 no. 14 3893-3902
    Species:Mouse
  12. Lan?a T, Costa MF, Gon?alves-Sousa N, et al. Protective Role of the Inflammatory CCR2/CCL2 Chemokine Pathway through Recruitment of Type 1 Cytotoxic gammadelta T Lymphocytes to Tumor Beds. J Immunol. 2013 May 17.
    Species:Mouse
    Sample Type:Tissue Lysate

More references are listed per product on the individual product pages.

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