Multiplex ELISA Cytokine Array: Quantibody®

Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Quantibody^® is an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types. Quantibody combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array. With this platform, only 50 µl of sample is needed for quantification of up to 40 proteins in quadruplicate, making this array 80 times more efficient than traditional ELISA.

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Quantiative Proteomics Services

High-Density Multiplex ELISA Full Testing Services

Ship us your samples (5 sample minimum) and our experienced service department will use our GLP-compliant Quantibody array platform to quantitatively detect up to 1200 human, 640 mouse, or 282 rat proteins and return to you a full report of your results. Click the button below to learn more.

Quantitative Proteomics Services

Quantibody Features

Get 4x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with 16 identical antibody arrays (also called "subarrays"). Within each subarray, the capture antibodies along with controls are spotted in quadruplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 14 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

Features

Less sample, more data: only 50 ul of sample is needed for quantification of up to 40 cytokines
4-hour processing time
More cost-effective than ELISA
No interference between capture antibodies (unlike bead-based multiplex assays)
No dedicated equipment required
Customizable - create a custom array from our list of targets

Contents of kit

Glass Slide/s
Sample Diluent
Lyophilized protein standard mix
Detection antibody cocktail
Streptavidin-Fluorescent dye
Wash buffer
Manual

*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

How It Works

Like a traditional sandwich-based ELISA, Quantibody uses a matched pair of antibodies for target protein detection. A panel of capture antibodies is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

To quantify target protein concentrations, the array-specific protein standards, whose concentrations have been predetermined, are provided to generate an 8-point standard curve of each target protein. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.

Scanning & Analysis

See our Laser Scanner Specifications Guide. If you do not have access to a scanner, RayBiotech now provides a free slide scanning and data extraction service. Simply ship us your slides and we will scan them and send you the raw data extracted from the image.

Need help analyzing all that data? Copy and paste your data into the RayBiotech Q-Analyzer Tool specific for your array. The Q-Analyzer will automatically plot the standard curve for each analyte as well as perform background subtraction and normalization.

Click the button below to learn about the free data extraction or full scanning and data analysis services offered by RayBiotech.

Array Scanning & Analysis Services

Research Applications

High-throughput profiling of cytokine expression
Validation of semi-quantitative antibody array results
Identifying potential molecular targets for drug development
Identifying the molecular mechanisms of drug action
Identifying crucial factors involved in disease processes
Discovering biomarkers for disease management
Discovering expression patterns for molecular classification of diseases

Product Citations

Sheedy, Frederick J., et al. "CD36 coordinates NLRP3 inflammasome activation by facilitating intracellular nucleation of soluble ligands into particulate ligands in sterile inflammation." Nature immunology 14.8 (2013): 812. [view publication]
Product: Mouse Inflammation Array Q1
Chappert, Pascal, et al. "Specific gut commensal flora locally alters T cell tuning to endogenous ligands." Immunity 38.6 (2013): 1198-1210. [view publication]
Product: Mouse Cytokine Array Q5
Zingariello, Maria, et al. "Characterization of the TGF-β1 signaling abnormalities in the Gata1 low mouse model of myelofibrosis." Blood 121.17 (2013): 3345-3363. [view publication]
Product: Mouse Cytokine Array Q1
Feng W., Madajka M., Kerr B., Mahabeleshwar G., White S., Byzova T. A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing. Blood April 7, 2011 vol. 117 no. 14 3893-3902 [view publication]
Product: Custom Quantibody® Mouse Array | Custom Quantibody® Human Array
Giroux, Martin, et al. "SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage." Blood 117.5 (2011): 1734-1744. [view publication]
Product: Custom Quantibody® Mouse Array
Schwartz S., Wongvipat J., Trigwell C., et al. Feedback Suppression of PI3Ka Signaling in PTEN-Mutated Tumors Is Relieved by Selective Inhibition of PI3Kb. Cancer Cell. 2015 Jan 12;27(1):109-22. doi: 10.1016/j.ccell.2014.11.008 [view publication]
Product: Human IGF Signaling Array Q1
Kränkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease." Circulation (2012): CIRCULATIONAHA-112. [view publication]
Product: Human Cytokine Array Q1
Jonnalagadda D., et al. Platelet secretion is kinetically heterogeneous in an agonist-responsive manner. December 20, 2012; Blood: 120 (26). http://dx.doi.org/10.1182/blood-2012-07-445080 [view publication]
Product: Custom Quantibody® Human Array
Palomero J., Vegilante M., Rodriguez M., et al. SOX11 promotes tumor angiogenesis through transcriptional regulation of PDGFA in mantle cell lymphoma. Blood. 2014 Oct 2;124(14):2235-47. doi: 10.1182/blood-2014-04-569566. [view publication]
Product: Custom Quantibody® Human Array
Feng, Weiyi, et al. "A novel role for platelet secretion in angiogenesis: mediating bone marrow-derived cell mobilization and homing." Blood 117.14 (2011): 3893-3902. [view publication]
Product: Custom Quantibody® Human Array
Moicsko K., Bordry N., Gfeller D., et al. Baseline serum predictors of clinical response to CTLA4 inhibitor therapy in melanoma patients. Journal of Clinical Oncology 33, no. 15_suppl (May 20 2015) 3025-3025 [view publication]
Product: Human Cytokine Array Q440