Quantibody® is an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types. Quantibody combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array. With this platform, only 50 µl of sample is needed for quantification of up to 40 proteins in quadruplicate, making this array 80 times more efficient than traditional ELISA.
Ship us your samples (5 sample minimum) and our experienced service department will use our GLP-compliant Quantibody array platform to quantitatively detect up to 1200 human, 640 mouse, or 282 rat proteins and return to you a full report of your results. Click the button below to learn more.
Each 75mm x 25mm glass slide is spotted with 16 identical antibody arrays (also called "subarrays"). Within each subarray, the capture antibodies along with controls are spotted in quadruplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 14 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.
*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Like a traditional sandwich-based ELISA, Quantibody uses a matched pair of antibodies for target protein detection. A panel of capture antibodies is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.
To quantify target protein concentrations, the array-specific protein standards, whose concentrations have been predetermined, are provided to generate an 8-point standard curve of each target protein. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.
See our Laser Scanner Specifications Guide. If you do not have access to a scanner, RayBiotech now provides a free slide scanning and data extraction service. Simply ship us your slides and we will scan them and send you the raw data extracted from the image.
Need help analyzing all that data? Copy and paste your data into the RayBiotech Q-Analyzer Tool specific for your array. The Q-Analyzer will automatically plot the standard curve for each analyte as well as perform background subtraction and normalization.
Click the button below to learn about the free data extraction or full scanning and data analysis services offered by RayBiotech.
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