Quantibody: Multiplex ELISA Array

Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Quantibody^® is an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types. Quantibody^® combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array.

With this platform, only 50 µl of sample is needed for quantification of up to 40 proteins in quadruplicate, making this array 80 times more efficient than traditional ELISA.

For a comparison & overview of all our antibody array systems, click here.

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Quantiative Proteomics Services

High-Density Quantibody Full Testing Services

Ship us your samples (5 sample minimum) and our experienced service department will use our GLP-compliant Quantibody^® array platform to quantitatively detect up to 1000 human, 640 mouse, or 67 rat proteins and return to you a full report of your results. Click the button below to learn more.

Quantitative Proteomics Services

Quantibody Features

Get 4x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with 16 identical antibody arrays (also called “subarrays”). Within each subarray, the capture antibodies along with controls are spotted in quadruplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 14 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

Features

Less sample, more data: only 50 ul of sample is needed for quantification of up to 40 cytokines
4-hour processing time
More cost-effective than ELISA
No interference between capture antibodies (unlike bead-based multiplex assays)
No dedicated equipment required
Customizable – create a custom array from our list of targets

Contents of kit

Glass Chip with antibody arrays
Sample Diluent
Lyophilized protein standard mix
Detection antibody cocktail
Streptavidin-Fluorescent dye
Wash buffer
Manual

*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

How It Works

Like a traditional sandwich-based ELISA, Quantibody uses a matched pair of antibodies for target protein detection. A panel of capture antibodies is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

To quantify target protein concentrations, the array-specific protein standards, whose concentrations have been predetermined, are provided to generate an 8-point standard curve of each target protein. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Quantibody slide scanning and data extraction service is now FREE for a limited time only!

Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Q-Analyzer Tool specific for your array. The Q-Analyzer will automatically plot the standard curve for each analyte as well as perform background subtraction and normalization.

Research Applications

High-throughput profiling of cytokine expression
Validation of semi-quantitative antibody array results
Identifying potential molecular targets for drug development
Identifying the molecular mechanisms of drug action
Identifying crucial factors involved in disease processes
Discovering biomarkers for disease management
Discovering expression patterns for molecular classification of diseases

Glass Slide Microarray Technique

References

Bigelman E., Cohen L., Aharon-Hananel G., et al. Pathological presentation of cardiac mitochondria in a rat model for chronic kidney disease. PLoS One. 2018 Jun 11;13(6):e0198196. doi: 10.1371/journal.pone.0198196 [view publication]
Product: Rat Inflammation Array Q1
Sample Type: Plasma
Dillman R., Cornforth A., Nistor G., et al. Randomized phase II trial of autologous dendritic cell vaccines versus autologous tumor cell vaccines in metastatic melanoma: 5-year follow up and additional analyses. J Immunother Cancer. 2018 Mar 6;6(1):19. doi: 10.1186/s40425-018-0330-1. [view publication]
Products: Human Cytokine Array Q4000
Sample Type: Serum
Izrael M., Slutsky SG., Admoni T., et al. Safety and efficacy of human embryonic stem cell-derived astrocytes following intrathecal transplantation in SOD1G93A and NSG animal models. Stem Cell Res Ther. 2018 Jun 6;9(1):152. doi: 10.1186/s13287-018-0890-5. [view publication]
Products: Human Kiloplex Quantitative Proteomics Array
Sample Type: Conditioned Media
Leung GP., Feng T., Sigoillot FD., et al. Hyperactivation of MAPK Signaling Is Deleterious to RAS/RAF-mutant Melanoma. Mol Cancer Res. 2018 Sep 10. doi: 10.1158/1541-7786.MCR-18-0327 [view publication]
Products: Human Kiloplex Quantitative Proteomics Array
Sample Type: Conditioned Media
Liao Y., Ivanova L., Zhu H., et al. Cord Blood-Derived Stem Cells Suppress Fibrosis and May Prevent Malignant Progression in Recessive Dystrophic Epidermolysis Bullosa. Stem Cells. 2018 Sep 24. doi: 10.1002/stem.2907. [view publication]
Product: Human Inflammation Array Q3
Sample Type: Conditioned Media
Platonov M., Borovjagin AV., Kaverina N., et al. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Lett. 2018 Mar 28;417:75-88. doi: 10.1016/j.canlet.2017.12.024. [view publication]
Products: Human Kiloplex Quantitative Proteomics Array
Sample Type: Conditioned Media
Vincent FB., Nim HT., Lee JPW., et al. Effect of storage duration on cytokine stability in human serum and plasma. Cytokine. 2018 Jun 14. pii: S1043-4666(18)30257-6. doi: 10.1016/j.cyto.2018.06.009. [view publication]
Products: Custom Quantibody^® Human Array
Sample Type: Serum
Duncan H., Smith A., Flemming G., et al. Release of bio-active dentine extracellular matrix components by histone deacetylase inhibitors (HDACi). Int Endod J. 2017 Jan;50(1):24-38. doi: 10.1111/iej.12588. [view publication]
Product: Human Growth Factor Array Q1
Sample Type: Conditioned Media
Erikson J., Valente A., Mummidi S., et al. Targeting TRAF3IP2 by genetic and interventional approaches inhibits ischemia/reperfusion-induced myocardial injury and adverse remodeling. J Biol Chem. 2017 Feb 10;292(6):2345-2358. doi: 10.1074/jbc.M116.764522. [view publication]
Product: Mouse Cytokine Array Q4000
Sample Type: Tissue Lysate
Fischer G., Wang F., Xiang H., et al. Inhibition of neuropathic hyperalgesia by intrathecal bone marrow stromal cells is associated with alteration of multiple soluble factors in cerebrospinal fluid. Exp Brain Res. 2017 Sep;235(9):2627-2638. doi: 10.1007/s00221-017-5000-x. [view publication]
Products: Rat Cytokine Array Q67
Sample Type: CSF
He J., Zhao J., Peng X., et al. Molecular Mechanism of MiR-136- 5p Targeting NF-κB/A20 in the IL-17- Mediated Inflammatory Response after Spinal Cord Injury. Cell Physiol Biochem. 2017;44(3):1224-1241. doi: 10.1159/000485452 [view publication]
Product: Rat Cytokine Array Q3
Sample Type: Conditioned Media
Himburg H., Doan P., Quarmyne M., et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med. 2017 Jan;23(1):91-99. doi: 10.1038/nm.4251. [view publication]
Products: Mouse Cytokine Array Q2000
Sample Type: Bone marrow fluid
Luck C., Demarco V., Mahmood A., et al. Differential Regulation of Cardiac Function and Intracardiac Cytokines by Rapamycin in Healthy and Diabetic Rats. Oxid Med Cell Longev. 2017;2017:5724046. doi: 10.1155/2017/5724046 [view publication]
Products: Rat Cytokine Array Q67
Sample Type: Tissue Lysate
Moicsko K., Bordry N., Gfeller D., et al. Baseline serum predictors of clinical response to CTLA4 inhibitor therapy in melanoma patients. Journal of Clinical Oncology 33, no. 15_suppl (May 20 2015) 3025-3025 [view publication]
Product: Human Cytokine Array Q440
Sample Type: Serum
Reategui E., Fatemeh J., Khankhel A., et al. Microscale arrays for the profiling of start and stop signals coordinating human-neutrophil swarming. Nature Biomedical Engineering 1, Article number: 0094 (2017) doi:10.1038/s41551-017-0094 [view publication]
Products: Human Chemokine Array Q1 | Human Cytokine Array Q4 | Human Cytokine Array Q5 | Human Cytokine Array Q6 | Human Cytokine Array Q7 | Human Cytokine Array Q8 | Human Cytokine Array Q9 | Human Growth Factor Array Q1 | Human Inflammation Array Q3 | Human Receptor Array Q1
Sample Type: Conditioned Media