Quantibody: Multiplex ELISA Array

Quantitative, Sandwich-based, Glass Slide Antibody Arrays

Quantibody^® is an array-based multiplex ELISA system for simultaneous quantitative measurement of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins in a wide variety of sample types. Quantibody^® combines the high specificity and sensitivity of ELISA with the high throughput of the glass chip-based array.

With this platform, only 50 µl of sample is needed for quantification of up to 40 proteins in quadruplicate (0.5 ml for the 400 target Quantibody array), making this array 80 times more efficient than traditional ELISA.

For a comparison & overview of all our antibody array systems, click here.

Compare Quantibody Protein Lists Customize Your Array Browse Quantibody Arrays

Quantiative Proteomics Services

High-Density Quantibody Full Testing Services

Ship us your samples (5 sample minimum) and our experienced service department will use our GLP-compliant Quantibody^® array platform to quantitatively detect up to 1000 human, 200 mouse, or 67 rat proteins and return to you a full report of your results. Click the button below to learn more.

Quantitative Proteomics Services

Quantibody Features

Get 4x the data, plus high throughput

Each 75mm x 25mm glass slide is spotted with 16 identical antibody arrays (also called “subarrays”). Within each subarray, the capture antibodies along with controls are spotted in quadruplicate. The slide comes with a 16-well removable gasket and chamber assembly that allows for simultaneous processing of up to 14 samples per slide. To achieve even higher throughput, 4 slides can be nested into a tray which matches a standard microplate, allowing for automated processing of 64 arrays simultaneously. The latest automated liquid handling work stations can accommodate several trays at once, allowing hundreds of samples to be processed per day.

Features

Less sample, more data: only 50 ul of sample is needed for quantification of up to 40 cytokines
4-hour processing time
More cost-effective than ELISA
No interference between capture antibodies (unlike bead-based multiplex assays)
No dedicated equipment required
Customizable – create a custom array from our list of targets

Contents of kit

Glass Chip with antibody arrays
Sample Diluent
Lyophilized protein standard mix
Detection antibody cocktail
Streptavidin-Fluorescent dye
Wash buffer
Manual

*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

How It Works

Like a traditional sandwich-based ELISA, Quantibody uses a matched pair of antibodies for target protein detection. A panel of capture antibodies is printed in multiple identical arrays on a standard slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are then visualized using a fluorescence laser scanner.

To quantify target protein concentrations, the array-specific protein standards, whose concentrations have been predetermined, are provided to generate an 8-point standard curve of each target protein. By comparing signals from unknown samples to the standard curve, the unknown cytokine concentration in the samples will be determined.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

RayBiotech Quantibody slide scanning and data extraction service is now FREE for a limited time only!

Simply ship us your slide/s and we will scan the slide/s and send you the raw data extracted from the image. Click to learn more

Need help analyzing all that data? Copy and paste your data into the RayBiotech Q-Analyzer Tool specific for your array. The Q-Analyzer will automatically plot the standard curve for each analyte as well as perform background subtraction and normalization.