Human Angiogenesis Array Q1

Quantibody® Human Angiogenesis Array 1 Kit. Detects 10 Human Angiogenic Factors. Suitable for all liquid sample types.

$285.83
QAH-ANG-1-1
+

Antigen Information

Gene Symbols:
  • ANG
  • ANGPT2
  • EGF
  • FGF2
  • HBEGF
  • HGF
  • LEP
  • PDGFB
  • PGF
  • VEGFA

Assay Format

Pathway:
  • HER/ErbB Signaling
Number of Targets Detected:
10
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Angiogenin
Angiopoietin-2
EGF
bFGF
HB-EGF
HGF
Leptin
PDGF-BB
PLGF
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Angiogenesis Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Zheng, Yi, et al. "Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis." Advanced healthcare materials (2016).
    Species: Human
    Sample type: Conditioned Media
  2. Villar C., Zhao X., Livi C., Cochran D. The Effect of Living Cellular Sheets on the Angiogenic Potential of Human Microvascular Endothelial Cells. Journal of Periodontology Posted online on January 16, 2015. doi:10.1902/jop.2015.140362
    Species: Human
    Sample type: Conditioned Media
  3. Granner T, Maloney S, Antecka E, et al. 3,4 dihydroxyphenyl ethanol reduces secretion of angiogenin in human retinal pigment epithelial cells. Br J Ophthalmol. 2013 Mar;97(3):371-374.
    Species: Human
    Sample type: Conditioned Media
  4. Maloney, Shawn C., et al. "Expression of SIRT1 in choroidal neovascular membranes." Retina 33.4 (2013): 862-866.
    Species: Human
    Sample type: Conditioned Media
  5. Dao, Mo, et al. "Comparing the angiogenic potency of naïve marrow stromal cells and Notch-transfected marrow stromal cells." Journal of translational medicine 11.1 (2013): 81.
    Species: Human
    Sample type: Conditioned Media
  6. Logan, Patrick, Julia Burnier, and Miguel N. Burnier Jr. "Vascular endothelial growth factor expression and inhibition in uveal melanoma cell lines." ecancermedicalscience 7 (2013).
    Species: Human
    Sample type: Conditioned Media
  7. Landazuri N., Levit R., Joseph G., et al. Alginate microencapsulation of human mesenchymal stem cells as a strategy to enhance paracrine-mediated vascular recovery after hindlimb ischaemia. J Tissue Eng Regen Med. 2016 Mar;10(3):222-32. doi: 10.1002/term.1680.
    Species: Human
    Sample type: Extracellular Vesicles
  8. Numasawa Y., et al. Treatment of Human Mesenchymal Stem Cells with Angiotensin Receptor Blocker Improved Efficiency of Cardiomyogenic Transdifferentiation and Improved Cardiac Function via Angiogenesis. Stem Cells. 2011;29:1405-1414. DOI:10.1002/stem.691
    Species: Human
    Sample type: Conditioned Media
  9. Shinmura D., et al. Pretreatment of Human Mesenchymal Stem Cells with Pioglitazone Improved Efficiency of Cardiomyogenic Transdifferentiation and Cardiac Function. STEM CELLS 2011;29:357-366. DOI:10.1002/stem.574
    Species: Human
    Sample type: Conditioned Media
  10. Rodríguez?Muñoz, Y., et al. "Peripheral blood monocyte subsets predict antiviral response in chronic hepatitis C." Alimentary pharmacology & therapeutics 34.8 (2011): 960-971.
    Species: Human
    Sample type: Conditioned Media

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Price for all $348.85

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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