Human Cytokine Array Q1

Quantibody® Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.

QAH-CYT-1-1
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Antigen Information

Gene Symbols:
CCL2 | CCL3 | CCL4 | CCL5 | CSF2 | CXCL1 | CXCL2 | CXCL3 | CXCL8 | IFNG   View more

Assay Format

Number of Targets Detected:
20
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 50 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
GM-CSF
GRO alpha/beta/gamma
IFN-gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-10
IL-12 p70
IL-13
IL-2
IL-4
IL-5
IL-6
IL-8
(CXCL8)
MCP-1
(CCL2)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MMP-9
RANTES
(CCL5)
TNF alpha
VEGF-A

Application Notes

Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Human Cytokine Array Q1 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Chen T., Li H., Lin J., et al. Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array. Tuberculosis (Edinb). 2016 Mar;97:108-17. doi: 10.1016/j.tube.2015.12.007.
    Species: Human
    Sample type: Serum
  2. Chen S., Zha X., Shi L., et al. Upregulated TCR? improves cytokine secretion in T cells from patients with AML. J Hematol Oncol. 2015; 8: 72. Published online 2015 Jun 18. doi: 10.1186/s13045-015-0170-0
    Species: Human
    Sample type: Conditioned Media
  3. Zhan Y., Zou S., Hua F., Li F., Ji L., Wang W., Ye Y., Sun L., Chen H., Cheng Y. High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia. Immunology Letters Volume 160, Issue 1, July 2014, Pages 33-38
    Species: Human
    Sample type: Serum
  4. Wu H., et al. Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus. Annals of Hematology (2014) 93:353-354
    Species: Human
    Sample type: Plasma
  5. Jiang, Weidong, Ruo-Pan Huang, and Ruochun Huang. "Protein Expression Profiling by Antibody Array Analysis with Use of Dried Blood Spot Samples on Filter Paper." Journal of Immunological Methods. ScienceDirect, 25 Nov. 2013. Web.
    Species: Human
    Sample type: Serum
  6. Kränkel, Nicolle, et al. "Novel insights into the critical role of bradykinin and the kinin B2 receptor for vascular recruitment of circulating endothelial repair-promoting mononuclear cell subsets: alterations in patients with coronary disease." Circulation (2012): CIRCULATIONAHA-112.
    Species: Human
    Sample type: Conditioned Media
  7. Lönne, M., Lavrentieva, A., Walter, J. G., & Kasper, C. (2013). Analysis of oxygen-dependent cytokine expression in human mesenchymal stem cells derived from umbilical cord. Cell and tissue research, 353(1), 117-122.
    Species: Human
    Sample type: Cell Lysate
  8. Logan, Patrick, Julia Burnier, and Miguel N. Burnier Jr. "Vascular endothelial growth factor expression and inhibition in uveal melanoma cell lines." ecancermedicalscience 7 (2013).
    Species: Human
    Sample type: Conditioned Media
  9. Varnum S., Springer D., Chaffee M., et al. The effects of low-dose irradiation on inflammatory response proteins in a 3D reconstituted human skin tissue model. Radiat Res. 2012 Dec;178(6):591-9. doi: 10.1667/RR2976.1.
    Species: Human
    Sample type: Tissue Lysate
  10. Sharma, M., et al. "The potential use of Echinacea in acne: control of Propionibacterium acnes growth and inflammation." Phytotherapy Research25.4 (2011): 517-521.
    Species: Human
    Sample type: Conditioned Media
This kit has been used to quantify the cytokines in the serum from patients in the following paper: Circ Res. 2016 Nov 11;119(11):1226-1241. This assay is very sensitive.

Temple University
Directions easy to follow and they provide free scanning services. I also recommend the software.

University of Colorado Anschutz Medical Campus
Definitely one of the better cytokine arrays I've worked with.

Washington University in St. Louis
Instructions were very clear and straightforward; it was a relatively simple process for a first-time user. Plenty of buffers and diluents included, and free analyzing by Ray-biotech if you don't have a compatible scanner accessible.

Washington University in St. Louis
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Q-Analyzer Bundle Discount

70% off the Q-Analyzer tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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