One-step preparation of competent Escherichia coli using Transformation and Storage Solution (1× TSS) has been developed by Chung et al (1). 1× TSS is a cocktail of LB broth, PEG, DMSO and Mg2+. This solution allows rapid preparation of the competent cells from any bacterial strains with high transformation efficiency. The transformation procedure can be completed in 1.5 hours without 42°C heat-shock (2). Benefits to use TSS-competent cells:
Store at -20 °C or -80 °C in a freezer with a manual defrost. Don’t use or store 1× TSS near a flame because DMSO in 1× TSS is flammable!
Store at -20 °C or 4 °C.
Store at -20 °C.
No posts found
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.