Rabbit Anti-YWHAZ

Rabbit Anti-YWHAZ

$420.00
DS-PB-00014
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Antigen Information

Protein Name & Synonyms:
YWHAZ
Target Species:
  • Sheep

Antibody Specifications

Applications:
ELISA (dilution: , 1/3000), Immunohistology - Paraffin, Western Blotting
Preparation:
Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.
Species Reactivity:
Sheep, Bovine, Chicken, Human, Mouse, Rabbit, Rat

This product detects a band of approximately 30kDa in HEK293 cell lysates.

Specificity

This product recognizes the acetylated N-terminal of 14-3-3 zeta and the phosphorylated zeta isoform (also known as 14-3-3 delta) in all mammals. These are members of the 14-3-3 family which consists of 30kDa proteins that are involved in multiple protein kinase signaling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.

14-3-3 zeta is a susceptibility gene for paranoid schizophrenia and is overexpressed in the temporal cortex of cognitively impared Alzheimers patients. The protein binds yeast Sps1/Ste20-related kinase 1 (YSK1) and localizes to the golgi, possibly linking YSK1 signaling, protein transport, cell adhesion and migration. It co-localizes with epidermal growth factor receptor to the plasma membrane following epidermal growth factor signaling. The zeta isoform is involved in MAPKAPK2-mediated phosphorylation which may have a role in p38 MAPK-dependent inflammation. When bound to ADAM 22, 14-3-3 zeta is involved in cell adhesion. 14-3-3 zeta also interacts with cofilin, LIM-domain-containing protein kinase 1, protein kinase B/Akt and GPI alpha. 14-3-3 zeta can be phosphorylated at serine 184 to produce 14-3-3 delta.

This product may not react with recombinant proteins that are not N-acetylated.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Serum - liquid
Format Type:
Serum

Preparation

Antisera to anti 14-3-3 zeta/delta were raised by repeated immunisations of rabbits with highly purified antigen.

Immunogen:
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 zeta/delta.

Peptide sequence: Ac.MDKNELVQKAC
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.