Rabbit Anti-14-3-3

Rabbit Anti-14-3-3

DS-PB-00003
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Antigen Information

Protein Name & Synonyms:
14-3-3
Target Species:
  • Sheep

Antibody Specifications

Applications:
Immunoprecipitation, Immunohistology - Paraffin, Western Blotting
Preparation:
Antisera to sheep 14-3-3 protein were raised by repeated immunisations of rabbit with highly purified antigen.
Species Reactivity:
Sheep, Amphibia, Bovine, Chicken, Fungal, Human, Mouse, Plants, Rat, Reptile

This product detects a band of approximately 30kDa in HEK293 cell lysates.

Immunohistology:
This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Specificity

This product recognizes all isoforms of the 14-3-3 protein family. The 14-3-3 family consists of 30kD proteins that are involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Serum - liquid
Format Type:
Serum

Preparation

Antisera to sheep 14-3-3 protein were raised by repeated immunisations of rabbit with highly purified antigen.

Immunogen:
Mixture of synthetic peptides corresponding to all isoforms of sheep 14-3-3.
Preservative:
0.09%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.