Rabbit anti-AKTIP Antibody

AKTIP Polyclonal Antibody

144-11822-100
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Antigen Information

Accession Number:
  • Q9H8T0
Gene ID:
  • 64400
Gene Symbols:
  • AKTIP
Protein Name & Synonyms:
AKTIP, FT1, FTS, AKT-interacting protein
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW
33kDa
Observed MW
40kDa

Immunogen

A synthetic peptide of human AKTIP

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using AKTIP antibody (144-011822) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - AKTIP antibody (144-011822)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.