Rabbit anti-ALDH1A1 Antibody

ALDH1A1 Polyclonal Antibody

$200.00
144-01802-50
+

Antigen Information

Accession Number:
  • P00352
Gene ID:
  • 216
Gene Symbols:
  • ALDH1A1
Protein Name & Synonyms:
ALDH1A1, ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, retinal dehydrogenase 1
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IHC
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
54kDa
Observed MW
55kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (144-01802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 20s.
Western blot - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded rat brain using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded rat kidney using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded human prostate using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (144-01802) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALDH1A1 antibody (144-01802)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.