Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (144-06240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug ALKBH1 antibody (144-06240). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (144-06240) at a dilition of 1:1000.
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