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Rabbit anti-ALKBH1 Antibody

ALKBH1 Polyclonal Antibody

$200.00  
144-06240-50
+ −

Antigen Information

Accession Number:
  • Q13686
Gene ID:
  • 8846
Gene Symbols:
  • ALKBH1
Protein Name & Synonyms:
ALKBH1, ABH, ABH1, ALKBH, alkB, hABH, nucleic acid dioxygenase ALKBH1
Target Species:
  • Human

Antibody Specifications

Applications:
WB, IP
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:200

Format

Calculated MW
43kDa
Observed MW
44kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 110-389 of human ALKBH1 (NP_006011.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ALKBH1 antibody (144-06240) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Western blot - ALKBH1 Polyclonal Antibody (144-06240)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug ALKBH1 antibody (144-06240). Western blot was performed from the immunoprecipitate using ALKBH1 antibody (144-06240) at a dilition of 1:1000.
Immunoprecipitation - ALKBH1 Polyclonal Antibody (144-06240)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.