Rabbit anti-APITD1 Antibody

APITD1 Polyclonal Antibody
CODE:  144-08293

Protein Information

Accession Number:
  • Q8N2Z9
Gene ID:
  • 378708
Gene Symbols:
  • APITD1
Protein Name & Synonyms:
CENPS, APITD1, CENP-S, FAAP16, MHF1, centromere protein S
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene was identified in the neuroblastoma tumor suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumors, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway. The protein is a component of multiple complexes, including the Fanconi anemia (FA) core complex, the APITD1/CENPS complex, and the CENPA-CAD (nucleosome distal) complex. Known functions include an involvement with chromatin associations of the FA core complex, and a role in the stable assembly of the outer kinetochore. Alternative splicing of this gene results in multiple transcript variants. Naturally occurring read-through transcripts also exist between this gene and the downstream cortistatin (CORT) gene, as represented in GeneID:100526739. An APITD1-related pseudogene has been identified on chromosome 7.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
9kDa/15kDa/19kDa
Observed MW
16kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human APITD1 (NP_954988.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using APITD1 antibody (144-08293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - APITD1 Polyclonal Antibody (144-08293)

Expiration:
12 months from the date of shipment when stored properly.