Rabbit anti-ATRIP Antibody

ATRIP Polyclonal Antibody

$200.00  
144-07139-50
+

Antigen Information

Accession Number:
  • Q8WXE1
Gene ID:
  • 84126
Gene Symbols:
  • ATRIP
Protein Name & Synonyms:
ATRIP, ATR-interacting protein
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IP
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse
Clonality:
Polyclonal
Isotype:
IgG

Background

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IP 1:50 - 1:200

Format

Calculated MW
72kDa/76kDa/83kDa/85kDa
Observed MW
86kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ATRIP antibody (144-07139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Western blot - ATRIP antibody (144-07139)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (144-07139). Western blot was performed from the immunoprecipitate using ATRIP antibody (144-07139) at a dilition of 1:1000.
Immunoprecipitation - 1ug ATRIP antibody (144-07139)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.