Protein Name & Synonyms:
ATRIP, ATR-interacting protein
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IP 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
Western blot analysis of extracts of various cell lines, using ATRIP antibody (144-07139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (144-07139). Western blot was performed from the immunoprecipitate using ATRIP antibody (144-07139) at a dilition of 1:1000.