Protein Name & Synonyms:
AURKA, AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7, aurora kinase A
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
WB 1:500 - 1:2000
A synthetic peptide of human AURKA
Western blot analysis of extracts of various cell lines, using AURKA antibody (144-02121) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.