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Antigen Information
Protein Name & Synonyms:
BAK / BAK1
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistology - Frozen, Western Blotting
Concentration:
1.0mg/ml
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
This product detects a band of approximately 28kDa in L1210 cell lysate
Specificity
This product recognizes the Bcl-2 homologous antagonist/killer (Bak), an oligomeric protein localised to the outer mitochondrial membrane and the endoplasmic reticulum of healthy cells. Bak is a pro-apoptotic Bcl-2 family member similar to Bax with which it is thought to share significant homology. Mcl-1 and Bcl-xL interact with Bak and prevent the activation of Bak in healthy cells. Upon cytotoxic signalling, Mcl-1- and Bcl-xL-binding proteins (such as Noxa and Bad) displace Bak and apoptosis is induced. When activated, Bak (and Bax) permeabilises the outer membrane of the mitochondria or the ER to release proapoptogenic factors such as cytochrome c.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antiserum to BAK (NT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen:
A peptide corresponding to 13 amino acids near the amino terminus of human BAK.
Preservative:
0.02% Sodium Azide (NaN3)
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
